Investigation of Ferritin Desorption from Gold Initiated by In Situ pH-Change

• Authors: V. Poór , O. Kasyutich , K. Hallam , W. Schwarzacher
• Languages of publication: EN

Abstracts

EN

One of the main questions regarding protein adsorption is about the reversibility of the adsorption process. To get a deeper understanding of this, adsorption of ferritin on Au was studied by quartz crystal microbalance and the pH of the buffer was changed in situ between two values that favour adsorption by different amounts. We found that although some ferritin desorbs from Au, the desorption is incomplete. When the desorption reached a constant value, we returned to the original conditions and investigated the readsorption. Our experiments show that the adsorption of ferritin onto Au is a partly reversible process. We found that for different initial ferritin coverages the proportion of ferritin that had been subsequently desorbed was approximately constant.

Keywords

EN

81.07.Nb; 87.14.E-; 87.15.R-

Discipline

• 87.15.R-: Reactions and kinetics(see also 82.39.-k Chemical kinetics in biological systems in physical chemistry)
• 87.14.E-: Proteins
• 81.07.Nb: Molecular nanostructures

• Journal: Acta Physica Polonica A
• Year: 2010
• Volume: 117
• Issue: 2
• Pages: 394-396

Dates

published

2010-02

Contributors

author

V. Poór

• Department of Physics, University of Bristol, Bristol, UK

author

O. Kasyutich

• Department of Physics, University of Bristol, Bristol, UK

author

K. Hallam

• Interface Analysis Centre, University of Bristol, Bristol, UK

author

W. Schwarzacher

• Department of Physics, University of Bristol, Bristol, UK

References

• 1. F. Caruso, et al., J. Colloid Interface Sci. 186, 129 (1997)
• 2. A.G. Hemmersam, et al., J. Phys. Chem. C 112 4180 (2008)
• 3. K. Uto, et al., J. Mater. Chem. 18 3876 (2008)
• 4. E. Ferapontova, et al., Bioelectrochemistry 55 127 (2002)
• 5. M.A. Cole, et al., Biomaterials 30 1827 (2009)
• 6. P.R. Tassel, et al., J. Chem. Phys. 106 761 (1997)
• 7. M.J. Mura-Galelli, et al., Biochemistry 88 5557 (1991)
• 8. H. Liu, et al., Colloids Surf. B 5 35 (1995)
• 9. J. Talbot, Adsorption 2 89 (1996)
• 10. G. Thomas, Medicinal Chemistry, Wiley, New York 2000
• 11. G. Sauerbrey, Z. Phys. 155 206 (1959)
• 12. S.T. Silk, et al., J. Biol. Chem. 251 6963 (1976)

Identifiers

DOI

10.12693/APhysPolA.117.394