PL EN


Preferences help
enabled [disable] Abstract
Number of results
2009 | 116 | S | S-156-S-159
Article title

AFM Force Spectroscopy and Steered Molecular Dynamics Simulation of Protein Contactin 4

Authors
Content
Title variants
Languages of publication
EN
Abstracts
EN
We use a single molecule atomic force spectroscopy combined with the steered molecular dynamics simulation to determine a mechanical behavior of neural cell adhesion protein contactin during its unfolding. Force curves typical for modular proteins were observed, showing at most four unfolding peaks. The analysis of force spectra performed within worm-like chain model of polymer elasticity showed the presence of three unfolding lengths. Small plateaus, most likely resulting from forced transitions within domains were observed for the first time. Steered molecular dynamics simulations help to determine atomistic picture of domain unfolding.
Keywords
EN
Publisher

Year
Volume
116
Issue
S
Pages
S-156-S-159
Physical description
Dates
published
2009-12
Contributors
author
  • Institute of Physics, Nicolaus Copernicus University, Grudziądzka 5, 87-100 Toruń, Poland
References
  • 1. T. Kaneko-Goto, S. Yoshihara, H. Miyazaki, Y. Yoshihara, Neuron 57, 834 (2008)
  • 2. T. Fernandez, T. Morgan, N. Davis, A. Klin, A. Morris, A. Farhi, R.P. Lifton, M.W. State, Am. J. Human Gen. 74, 1286 (2004)
  • 3. J. Roohi, C. Montagna, D.H. Tegay, L.E. Palmer, C. DeVincent, J.C. Pomeroy, S.L. Christian, N. Nowak, E. Hatchwell, J. Med. Genet. 46, 176 (2009)
  • 4. G. Binnig, C.F. Quate, C. Gerber, Phys. Rev. Lett. 56, 930 (1986)
  • 5. E.L. Florin, V.T. Moy, H.E. Gaub, Science 264, 415 (1994)
  • 6. H. Clausen-Schaumann, M. Rief, C. Tolksdorf, H.E. Gaub, Biophys. J. 78, 1997 (2000)
  • 7. P.E. Marszalek, A.F. Oberhauser, Y.P. Pang, J.M. Fernandez, Nature 396, 661 (1998)
  • 8. G. Lee, W. Nowak, J. Jaroniec, Q. Zhang, P.E. Marszalek, Biophys. J. 87, 1456 (2004)
  • 9. M. Rief, M. Gautel, F. Oesterhelt, J.M. Fernandez, H.E. Gaub, Science 276, 1109 (1997)
  • 10. G. Lee, K. Abdi, Y. Jiang, P. Michaely, V. Bennett, P. Marszalek, Nature 440, 246 (2006)
  • 11. K. Lebed, M. Lekka, J. Lekki, W.M. Kwiatek, A. Dabrowska, J. Phys. Condens. Matter 18, 10157 (2006)
  • 12. J. Roohi, C. Montagna, D.H. Tegay, L.E. Palmer, C. DeVincent, J.C. Pomeroy, S.L. Christian, N. Nowak, E. Hatchwell, Brit. Med. J. 336, 414 (2008)
  • 13. M. Rabbi, P.E. Marszalek, Cold Spring Harbor Protocols 2007, pdb.prot4899, 2007
  • 14. J.L. Hutter, J. Bechhoefer, Rev. Sci. Instrum. 64, 1868 (1993)
  • 15. C. Bustamante, J.F. Marko, E.D. Siggia, S.B. Smith, Science 265, 1599 (1994)
  • 16. M.T. Nelson, W. Humphrey, A. Gursoy, A. Dalke, L.V. Kale, R.D. Skeel, K. Schulten, Int. J. High Perform. Comput. Appl. 10, 251 (1996)
  • 17. T. Schwede, J. Kopp, N. Guex, M.C. Peitsch, Nucleic Acids Res. 31, 3381 (2003)
  • 18. W. Nowak, P. Marszalek, in: Current Trends in Computational Chemistry, Ed. J. Leszczyński, World Scientific, Singapore 2005
  • 19. W. Humphrey, A. Dalke, K. Schulten, J. Mol. Graph. 14, 33 (1996)
  • 20. A.P. Wiita, S.R.K. Ainavarapu, H.H. Huang, J.M. Fernandez, Proc. Nat. Acad. Sci. 103, 7222 (2006)
  • 21. P. Marszalek, H. Lu, H. Li, M. Carrion-Vazquez, A. Oberhauser, K. Schulten, J. Fernandez, Nature 402, 100 (1999)
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-appv116ns43kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.