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2009 | 116 | 4 | 705-707
Article title

Ellipsometrical and SEM Study of Ferritin Adsorption οn a Gold Surface

Content
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Languages of publication
EN
Abstracts
EN
The adsorption of horse spleen ferritin on gold surface is studied with null ellipsometry and scanning electron microscopy. The high sensitivity and in situ advantage of ellipsometry make it very attractive for studies of ultrathin biomolecular layers. The influence of pH and protein concentration in solution on the adsorption kinetics was interpreted using ex situ scanning electron microscopy shots and computer modeling of the system "prism-gold film-adsorbed layer-solution". It seems that the first fast stage comprises nucleation process occurring at active sites and the following slow multistage process of a protein film growth. The data about adsorption kinetics of protein and the structure of the adsorbate layers obtained in this study enable understanding of such processes in real biological system, e.g. the protein adsorption on blood vessels and tissues in pathological processes.
Keywords
Year
Volume
116
Issue
4
Pages
705-707
Physical description
Dates
published
2009-10
References
  • 1. M. Wahlgren, T. Arnebrant, Trends Biotechnol. 9, 2001 (1991)
  • 2. Surface and Interfacial Aspects of Biomedical Polymers, Vol. 2, Protein Adsorption, Ed. J.D. Andrade, Plenum Press, New York 1985
  • 3. J.D. Andare, V. Hlady, Adv. Polym. Sci. 70, 1 (1986)
  • 4. B. Ivansson, I. Lundström, CDC Crit. Rev. Biocomp. 2, 1 (1986)
  • 5. H. Nygren, H. Arwin, S. Welin-Klinström, Colloids Surf. A Physicochem. Eng. Asp. 76, 87 (1993)
  • 6. H. Nygren, Biophys. J. 65, 1508 (1994)
  • 7. D.R. Weaver, W.G. Pitt, Biomaterials 13, 577 (1992)
  • 8. H.D. Jennissen, J. Chromatogr. 159, 71 (1978)
  • 9. I. Lundström, H. Elwing, J. Colloid Interface Sci. 136, 68 (1990)
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-appv116n492kz
Identifiers
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