Changes of the Electrode Surface Roughness Induced by High-Voltage Electric Pulses as Revealed by AFM

• Authors: R. Rodaitė-Riševičienė , G. Saulis , V. Snitka
• Languages of publication: EN

Abstracts

EN

The changes of the surface topography of stainless-steel and aluminium electrodes occurring due to the action of electric pulses which are utilized for cell electroporation, have been studied by using atomic force microscopy. The surfaces of the polished stainless-steel electrodes were smooth - the average roughness was 13-17 nm and the total roughness 140-180 nm. The total roughness of the aluminium electrodes was about 320 nm. After the treatment of the chambers filled with 154 mM NaCl solution by a series of short (20-40 μs), high-voltage (4 kV) pulses with the total dissolution charge of 0.20-0.26 A s/cm^{2}, the roughness of the surface of the electrodes has increased, depending on the total amount of the electric charge that has passed through the unit area of the electrode. Up to a two- and threefold increase of the surface roughness of the stainless-steel and aluminium anodes respectively was observed due to the dissolution of the anode material. Therefore, the use of high-voltage electric pulses leads to the increase of the inhomogeneity of the electric field at the electrode, which facilitates the occurrence of the electric breakdown of the liquid samples and causes non-equal treatment of each cell.

Keywords

EN

87.80.-y; 82.45.Fk; 68.37.Ps

Discipline

• 82.45.Fk: Electrodes
• 68.37.Ps: Atomic force microscopy (AFM)
• 87.80.-y: Biophysical techniques (research methods)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2009
• Volume: 115
• Issue: 6
• Pages: 1095-1097

Dates

published

2009-06

Contributors

author

R. Rodaitė-Riševičienė

• Vytautas Magnus University, 58 K. Donelaičio str., LT-44248 Kaunas, Lithuania
• Kaunas University of Technology, 65 Studentų str., LT-51369 Kaunas, Lithuania

author

G. Saulis

• Vytautas Magnus University, 58 K. Donelaičio str., LT-44248 Kaunas, Lithuania

author

V. Snitka

• Kaunas University of Technology, 65 Studentų str., LT-51369 Kaunas, Lithuania

References

• 1. J. Gehl, Acta Physiol. Scand. 177, 437 (2003)
• 2. G. Milazzo, Electrochemistry: Theoretical Principles and Practical Applications, Elsevier, Amsterdam 1963
• 3. J.W. Loomis-Husselbee, P.J. Cullen, R.F. Irvine, A.P. Dawson, Biochem. J. 277, 883 (1991)
• 4. T. Tomov, I. Tsoneva, Bioelectrochemistry 51, 207 (2000)
• 5. G. Saulis, R. Lape, R. Praneviciute, D. Mickevicius, Bioelectrochemistry 67, 101 (2005)
• 6. G. Binnig, C.F. Quate, C. Gerber, Phys. Rev. Lett. 56, 930 (1986)
• 7. G. Saulis, R. Rodaite-Riseviciene, V. Snitka, Bioelectrochemistry 70, 519 (2007)
• 8. I.E. Pol, W.G.C. van Arendonk, H.C. Mastwijk, J. Krommer, E.J. Smid, R. Moezelaar, Appl. Environ. Microbiol. 67, 1693 (2001)
• 9. M. Puc, S. Čorovic, K. Flisar, M. Petkovšek, J. Nastran, D. Miklavčič, Bioelectrochemistry 64, 113 (2004)

Identifiers

DOI

10.12693/APhysPolA.115.1095