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2006 | 109 | 3 | 273-278
Article title

Irradiation of Living Cells with Single Ions at the Ion Microprobe SNAKE

Content
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Languages of publication
EN
Abstracts
EN
The irradiation setup at the ion microprobe SNAKE is used to irradiate living cells with single energetic ions. The irradiation accuracy of 0.55μm and respectively 0.40μm allows to irradiate substructures of the cell nucleus. By the choice of ion atomic number and energy the irradiation can be performed with a damage density adjustable over more than three orders of magnitude. Immunofluorescence detection techniques show the distribution of proteins involved in the repair of DNA double-strand breaks. In one of the first experiments the kinetics of appearance of irradiation-induced foci in living HeLa cells was examined. In other experiments a new effect was detected which concerned the interaction between irradiation events performed at different time points within the same cell nucleus.
Keywords
Publisher

Year
Volume
109
Issue
3
Pages
273-278
Physical description
Dates
published
2006-03
received
2005-05-20
Contributors
author
  • Physik Department E12, Technische Universität, München, Germany
author
  • Department Biologie II, Ludwig-Maximilians-Universität, München, Germany
author
  • Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany
author
  • Department Biologie II, Ludwig-Maximilians-Universität, München, Germany
author
  • Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany
author
  • Physik Department E12, Technische Universität, München, Germany
author
  • Institut für Angewandte Physik und Messtechnik (LRT 2), Universität der Bundeswehr, München, Germany
author
  • Physik Department E12, Technische Universität, München, Germany
author
  • Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany
author
  • Department Biologie II, Ludwig-Maximilians-Universität, München, Germany
author
  • Institut für Angewandte Physik und Messtechnik (LRT 2), Universität der Bundeswehr, München, Germany
author
  • Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany
References
  • 1. P. Reichart, G. Datzmann, A. Hauptner, R. Hertenberger, C. Wild, G. Dollinger, Science, 306, 1537, 2004
  • 2. A. Hauptner, S. Dietzel, G.A. Drexler, P. Reichart, R. Krücken, T. Cremer, A.A. Friedl, G. Dollinger, Radiat. Environ. Biophys., 42, 237, 2004
  • 3. J.F. Ziegler, J.P. Biersack, http://www.srim.org
  • 4. ROOT (An Object-Orientated Data Analysis Framework), http://root.cern.ch
  • 5. V. Hable, Diploma thesis, TU München, Physik Department, 2004
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-appv109n305kz
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