Irradiation of Living Cells with Single Ions at the Ion Microprobe SNAKE

• Authors: A Hauptner , T Cremer , M Deutsch , S Dietzel , G Drexler , C Greubel , V Hable , R Krücken , R Löwe , H Strickfaden , G Dollinger , A Friedl
• Languages of publication: EN

Abstracts

EN

The irradiation setup at the ion microprobe SNAKE is used to irradiate living cells with single energetic ions. The irradiation accuracy of 0.55μm and respectively 0.40μm allows to irradiate substructures of the cell nucleus. By the choice of ion atomic number and energy the irradiation can be performed with a damage density adjustable over more than three orders of magnitude. Immunofluorescence detection techniques show the distribution of proteins involved in the repair of DNA double-strand breaks. In one of the first experiments the kinetics of appearance of irradiation-induced foci in living HeLa cells was examined. In other experiments a new effect was detected which concerned the interaction between irradiation events performed at different time points within the same cell nucleus.

Keywords

EN

29.27.Eg; 61.80.Jh; 87.16.Sr; 87.50.Gi

Discipline

• 29.27.Eg: Beam handling; beam transport
• 87.16.Sr: Chromosomes, histones
• 61.80.Jh: Ion radiation effects(for ion implantation, see 61.72.U-)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2006
• Volume: 109
• Issue: 3
• Pages: 273-278

Dates

published

2006-03

received

2005-05-20

Contributors

author

A Hauptner

• Physik Department E12, Technische Universität, München, Germany

author

T Cremer

• Department Biologie II, Ludwig-Maximilians-Universität, München, Germany

author

M Deutsch

• Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany

author

S Dietzel

• Department Biologie II, Ludwig-Maximilians-Universität, München, Germany

author

G Drexler

• Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany

author

C Greubel

• Physik Department E12, Technische Universität, München, Germany

author

V Hable

• Institut für Angewandte Physik und Messtechnik (LRT 2), Universität der Bundeswehr, München, Germany

author

R Krücken

• Physik Department E12, Technische Universität, München, Germany

author

R Löwe

• Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany

author

H Strickfaden

• Department Biologie II, Ludwig-Maximilians-Universität, München, Germany

author

G Dollinger

• Institut für Angewandte Physik und Messtechnik (LRT 2), Universität der Bundeswehr, München, Germany

author

A Friedl

• Strahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany

References

• 1. P. Reichart, G. Datzmann, A. Hauptner, R. Hertenberger, C. Wild, G. Dollinger, Science, 306, 1537, 2004
• 2. A. Hauptner, S. Dietzel, G.A. Drexler, P. Reichart, R. Krücken, T. Cremer, A.A. Friedl, G. Dollinger, Radiat. Environ. Biophys., 42, 237, 2004
• 3. J.F. Ziegler, J.P. Biersack, http://www.srim.org
• 4. ROOT (An Object-Orientated Data Analysis Framework), http://root.cern.ch
• 5. V. Hable, Diploma thesis, TU München, Physik Department, 2004

Identifiers

DOI

10.12693/APhysPolA.109.273