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2005 | 107 | 6 | 883-894
Article title

Fluorescence Decay Heterogeneity Model Based on Electron Transfer Processes in an Enzyme-Ligand Complex

Content
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Languages of publication
EN
Abstracts
EN
The models are described for complex fluorescence decay of tyrosine in proteins involving continuous distribution of fluorescence lifetimes and electron transfer processes. We introduce the analytical decay function with a power-like term, which provides good fits to highly complex fluorescence decays. Moreover, the power-like term in the proposed decay functions is a manifestation of so-called Tsallis nonextensive statistics and is suitable for description of the systems with long-range interactions, memory effect, as well as with fluctuations of the characteristic lifetime of fluorescence. The proposed decay functions were applied to analysis of fluorescence decays of tyrosine in a protein, i.e. the enzyme purine nucleoside phosphorylase from E. coli, free in aqueous solution and in the complex with formycin A (an inhibitor) and orthophosphate (a co-substrate), and demonstrated that both models reflect the enzyme-ligand interactions. Direct measure of heterogeneity of the enzyme systems is provided by a variance of fluorescence lifetime distribution. The possible number of deactivation channels and excited state mean lifetime can be easily derived without a priori knowledge of the complexity of studied system.
Keywords
EN
Year
Volume
107
Issue
6
Pages
883-894
Physical description
Dates
published
2005-06
received
2004-12-28
(unknown)
2005-02-07
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-appv107n606kz
Identifiers
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