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2003 | 104 | 3-4 | 373-380
Article title

Study of Probes and Substrates for Low Temperature Atomic Force Microscopy and Biological Applications

Content
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Languages of publication
EN
Abstracts
EN
The atomic force microscopy in ultrahigh vacuum and at low temperature demonstrated its excellent capability to reach atomic resolution. Nevertheless in the case of biological samples high resolution has been achieved only in very few cases. We demonstrated here the importance of the appropriate choice of probes and substrates in order to image DNA at low temperature with high resolution. We investigated properties of three types of cantilevers and they were studied by scanning electron microscopy as a function of temperature. A large bending of cantilevers, which were coated from both sides, was observed at low temperatures. Therefore uncoated cantilevers are strongly recommended for low temperature applications. Different methods for immobilization of DNA on the substrate are examined at low temperatures. First images of linear DNA on graphite at 82 K under ultrahigh vacuum conditions are presented.
Keywords
Year
Volume
104
Issue
3-4
Pages
373-380
Physical description
Dates
published
2003-09/10
received
2003-07-16
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-appv104n308kz
Identifiers
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