PL EN


Preferences help
enabled [disable] Abstract
Number of results
2003 | 104 | 3-4 | 321-326
Article title

Force Spectroscopy of Polyclonal and Monoclonal Anti-Bovine Serum Albumin Antibodies - BSA Complexes

Content
Title variants
Languages of publication
EN
Abstracts
EN
The specific interactions between bovine serum albumin and poly- or two monoclonal bovine serum albumin antibodies were studied using force spectroscopy mode of atomic force microscopy. The histograms of the unbinding forces for polyclonal bovine serum albumin antibodies are broad at high antibody concentrations (50 or 270 μg/ml) and narrow at low concentrations (10 or 27 μg/ml), while the histograms for monoclonal antibodies peak at well defined unbinding force. The peak unbinding force depends on the type of antibody and the antibody concentration. In this paper we described and characterized the passive adsorption and covalent immobilization of proteins for tip and sample preparation. Force spectroscopy could serve as a useful method for characterization of antigen-antibody interactions for measuring the specificity of an antibody or to assess the purity of a monoclonal antibody solution and to distinguish between different antibodies.
Keywords
EN
Contributors
  • Institut de Physique de la Matière Condensée, University of Lausanne, 1015 Lausanne, Switzerland
author
  • Institut de Physique de la Matière Condensée, University of Lausanne, 1015 Lausanne, Switzerland
References
  • 1. P. Hinterdorfer, W. Baumgartner, H.J. Gruber, K. Schilcher, H. Schindler, Proc. Natl. Acad. Sci. USA, 93, 3477, 1996
  • 2. J. Dammer, M. Hegner, D. Anselmetti, P. Wagner, D. Dreier, W. Huber, H.-J. Güntherodt, Biophys. J., 70, 2437, 1996
  • 3. J.P. Cleveland, S. Manne, D. Bocek, P.K. Hansma, Rev. Sci. Instrum., 64, 403, 1993
  • 4. J.L. Hutter, J. Bechhoefer, Rev. Sci. Instrum., 64, 1868, 1993
  • 5. U. Jonsson, G. Olofsson, M. Malmqvist, I. Ronnberg, Thin Solid Films, 124, 117, 1985
  • 6. E.T. Vandenberg, L. Bertilsson, B. Lieberg, K. Uvdal, R. Erlandsson, H. Elwing, I. Lundstrom, J. Colloid Interface Sci., 147, 322, 1991
  • 7. Y.L. Lyubchenko, A.A. Gall, L.S. Shlyakhtenko, R.E. Harrington, B.L. Jacobs, P.I. Oden, S.M. Lindsay, J. Biomol. Struct. Dyn., 10, 589, 1992
  • 8. Y.L. Lyubchenko, R.E. Blankenship, S.M. Lindsay, L.S. Shlyakhtenko, Scanning Microscopy, 10, 1997, 1996
  • 9. L.S. Shlyakhtenko, A.A. Gall, J.J. Weimer, D.D. Hawn, Y.L. Lyubchenko, Biophys. J., 77, 568, 1999
  • 10. E.T. Vandenberg, L. Bertilsson, B. Liedberg, K. Uvdal, R. Erlandsson, H. Elwing, I. Lundström, J. Colloid Interface Sci., 147, 103, 1991
  • 11. T. Vallant, H. Brunner, U. Mayer, P. Hoffmann, T. Leitner, R. Resch, G. Friedbacher, J. Phys. Chem. B, 102, 7190, 1998
  • 12. S. Kasas, B.M. Riederer, S. Catsicas, B. Cappella, G. Dietler, Rev. Sci. Instrum., 71, 2082, 2000
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-appv104n304kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.