Application of Mössbauer Spectroscopy in Study of Selected Biochemical Processes

• Authors: K. Burda , J. Stanek
• Languages of publication: EN

Abstracts

EN

The ^{57}Fe, ^{119}Sn, ^{129}I, and ^{151}Eu Mössbauer spectroscopy, scanning force microscopy, and optical fluorescence method were applied to study biological systems starting from porphyrins, through cytochromes and cell membranes until such a complex system as photosystem II. In Fe-porphyrin aggregates iron atoms are able to trap an electron exhibiting the mixed valence Fe^{3+}-Fe^{2+} relaxation process. In ironcytochrome c the presence of two different Fe^{3+} states are indicated, while in tincytochrome Sn appears in Sn^{4+} and Sn^{2+} states. From the temperature dependence of the mean square displacement of the resonance nuclei and from the diffusional broadening of the Mössbauer line it was possible to separate the vibrational, fast collective and slow collective motions in tinporphyrin and in iron- and tin-cytochrome c. The electronic state of iodine in oleic acid, the main constituent of cellular membranes, was determined. The molecular mechanism of triphenyltin interaction with membrane of red blood cells has been suggested and the model of haemolysis has been proposed. In photosystem II, Eu ions replacing calcium showed Eu^{3+} to Eu^{2+} transition after illumination with light, which points out the possible role of Ca^{2+} ions in electron transfer in the process of photosynthetic water splitting process.

Keywords

EN

82.80.Ej; 82.39.Jn; 82.35.Np; 82.39.Wj; 82.39.Rt

Discipline

• 82.39.Rt: Reactions in complex biological systems(see also 87.18.-h Biological complexity)
• 82.80.Ej: X-ray, Mössbauer, and other γ-ray spectroscopic analysis methods
• 82.35.Np: Nanoparticles in polymers(see also 81.07.-b Nanoscale materials and structures: fabrication and characterization)
• 82.39.Wj: Ion exchange, dialysis, osmosis, electro-osmosis, membrane processes
• 82.39.Jn: Charge (electron, proton) transfer in biological systems

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2003
• Volume: 103
• Issue: 5
• Pages: 499-509

Dates

published

2003-05

received

2003-01-15

Contributors

author

K. Burda

• H. Niewodniczanski Institute of Nuclear Physics, Radzikowskiego 152, 31-342 Kraków, Poland

author

J. Stanek

• M. Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland

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Identifiers

DOI

10.12693/APhysPolA.103.499