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2002 | 101 | 5 | 583-588
Article title

Complementarity of Neutron and Ultrahigh Resolution Synchrotron X-Ray Protein Crystallography Studies: Results with Concanavalin A at Cryo and Room Temperature

Content
Title variants
Languages of publication
EN
Abstracts
EN
The complementarity of synchrotron derived ultrahigh resolution X-ray and neutron protein crystallography is explored via an ensemble of plant lectin concanavalin A crystal structures. Thus a resume of a study of a cryo 0.94Å and a neutron (+X-ray) protein crystal 2.4Å structure at room temperature is made and these are then compared in their efficiency to determine the positions of the bound solvent atoms i.e. as hydrogens or deuteriums. First results are also presented of comparisons of two ultrahigh resolution protein crystal structures, the 0.94Å and a new 0.92Å structure. Thus the variability of the two cryo structures, at very fine detail, is described; this variability is in the multiple occupancies of side chains. Overall, one can see that a "complete" structure definition, with today's experimental capabilities, is possible and can include structure ensemble variations.
Keywords
EN
Year
Volume
101
Issue
5
Pages
583-588
Physical description
Dates
published
2002-05
received
2001-08-31
References
  • 1. D. Vitkup, D. Ringe, G.A. Petsko, M. Karplus, Nat. Struct. Biol., 7, 34, 2000
  • 2. A. Deacon, T. Gleichmann, A.J. Kalb (Gilboa), H. Price, J. Raftery, G. Bradbrook, J. Yariv, J.R. Helliwell, Faraday Trans., 93, 4305, 1997
  • 3. J. Habash, J. Raftery, S. Weisgerber, A. Cassetta, M. Lehmann, P. Hoghoj, C. Wilkinson, J.W. Campbell, J.R. Helliwell, Faraday Trans., 93, 4313, 1997
  • 4. J. Habash, J. Raftery, R. Nuttall, H.J. Price, C. Wilkinson, A.J. Kalb (Gilboa), J.R. Helliwell, Acta Crystallogr. D, 56, 541, 2000
  • 5. H.J. Price, Ph.D. thesis, University of Manchester, 1999
  • 6. E.H. Snell, N.E. Chayen, J.R. Helliwell, Biochemist, 21, 19, 1999
  • 7. A.J. Kalb (Gilboa), D. Myles, J. Habash, J. Raftery, J.R. Helliwell, J. Appl. Crystallogr., 34, 454, 2001
  • 8. J.R. Helliwell, C.C. Wilson, in preparation
  • 9. Report of the ESS Biology and Biotechnology Science Definition Group, 2001, Engelberg Workshop (copies available from john.helliwell@man.ac.uk)
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-appv101n502kz
Identifiers
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