Non-histone nuclear protein HMGN2 differently regulates the urothelium barrier function by altering expression of antimicrobial peptides and tight junction protein genes in UPEC J96-infected bladder epithelial cell monolayer

• Authors: Hanwen Tian , Junming Miao , Fumei Zhang , Feng Xiong , Feimei Zhu , Jinyu Li , Xiaoying Wang , Shanzhe Chen , Junli Chen , Ning Huang , Yi Wang
• Languages of publication: EN

Abstracts

EN

The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection. Further investigation showed that BECs 5637 monolayer, in which HMGN2 was over-expressed, had significantly increased permeability to J96. Our study systemically explored the regulatory roles of HMGN2 in BECs barrier function during UPEC infection and suggested different modulations of intracellular and paracellular routes through which UPEC invades the bladder epithelium.

Keywords

EN

HMGN2; UPEC; BECs 5637; bladder epithelium; tight junction; antimicrobial peptides

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 1
• Pages: 93-100

Dates

published

2018

received

2017-06-12

revised

2017-11-28

accepted

2017-12-26

(unknown)

2018-03-17

Contributors

author

Hanwen Tian

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China
• Department of Pharmacology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Junming Miao

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Fumei Zhang

• Experimental Center, Northwest University for Nationalities, Lanzhou 730030, China

author

Feng Xiong

• Department of Cardiology, The Third People's Hospital of Chengdu, Chengdu, China

author

Feimei Zhu

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Jinyu Li

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Xiaoying Wang

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Shanzhe Chen

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Junli Chen

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Ning Huang

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

author

Yi Wang

• Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China

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Identifiers

DOI

10.18388/abp.2017_1622