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2018 | 65 | 3 | 471-478
Article title

Immune response against HtrA proteases in children with cutaneous mastocytosis

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EN
Abstracts
EN
Mast cells play an important role in both, the innate and adaptive immunity, however, clonal proliferation of abnormal mast cells in various organs leads to mastocytosis. A skin variant of the disease, cutaneous mastocytosis (CM) is the most frequent form of mastocytosis in children. HtrA proteases are modulators of important cellular processes, including cell signaling and apoptosis, and are related to development of several pathologies. The above and the observation that mast cells constitutively release the HtrA1 protein, prompted us to investigate a possible involvement of the HtrA proteins in pediatric CM. Levels of the serum autoantibodies (IgG) against the recombinant HtrA proteins (HtrA1-4) in children with CM (n=36) and in healthy controls (n=62) were assayed. Anti-HtrA IgGs were detected using enzyme linked immunosorbent assay (ELISA) and Western-blotting. In the CM sera, levels of the anti-HtrA1 and anti-HtrA3 autoantibodies were significantly increased when compared to the control group, while the HtrA protein levels were comparable. No significant differences in the anti-HtrA2 IgG level were found; for the anti-HtrA4 IgGs lower levels in CM group were revealed. In healthy children, the IgG levels against the HtrA1, -3 and -4 increased significantly with the age of children; no significant changes were observed for the anti-HtrA2 IgG. Our results suggest involvement of the HtrA1 and HtrA3 proteins in pediatric CM; involvement of the HtrA4 protein is possible but needs to be investigated further. In healthy children, the autoantibody levels against HtrA1, -3 and -4, but not against HtrA2, increase with age.
Publisher

Year
Volume
65
Issue
3
Pages
471-478
Physical description
Dates
published
2018
received
2018-06-05
revised
2018-07-12
accepted
2018-07-16
(unknown)
2018-08-27
Contributors
author
  • Department of General and Medical Biochemistry, University of Gdańsk, Gdańsk, Poland
  • Department of General and Medical Biochemistry, University of Gdańsk, Gdańsk, Poland
  • Department of Dermatology, Venerology and Allergology Medical University of Gdańsk, Gdańsk, Poland
  • Department of Dermatology, Venerology and Allergology Medical University of Gdańsk, Gdańsk, Poland
author
  • Faculty of Health Sciences with Subfaculty of Nursing, Medical University of Gdańsk, Gdańsk, Poland
  • Department of Pediatrics, Gastroenterology, Hepatology and Nutrition, Medical University of Gdańsk, Gdańsk, Poland
author
  • Department of Occupational, Metabolic and Internal Diseases, Medical University of Gdańsk, Gdynia, Poland
author
  • Department of Geriatrics, Qilu Hospital, Shandong University Jinan, Shandong, China
  • Department of Geriatrics, Qilu Hospital, Shandong University Jinan, Shandong, China
  • Department of Geriatrics, Qilu Hospital, Shandong University Jinan, Shandong, China
  • Department of Geriatrics, Qilu Hospital, Shandong University Jinan, Shandong, China
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv65p471kz
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