Novel luminescent dyes for confocal laser scanning microscopy used in Trematoda parasite diagnostics

• Authors: Elena Kirilova , Sanita Kecko , Ligita Mežaraupe , Inese Gavarāne , Aleksandrs Pučkins , Ilona Mickeviča , Ilze Rubeniņa , Sergejs Osipovs , Andrejs Bulanovs , Mihails Pupiņš , Muza Kirjušina
• Languages of publication: EN

Abstracts

EN

Benzanthrone derivates are now widely used in many industrial and scientific applications as dyes for polymers and textiles. In biochemical, biomedical and diagnostics investigations benzanthrone dyes are used as a lipophilic fluorescent probe since many benzanthrone derivates demonstrate bright fluorescence and they have ability to intercalate between membrane lipids. The aim of research presented here was to assess the luminescence ability of benzanthrone derivatives using microscopic visualization of biological objects. Accordingly, specimens of freshwater trematodes: Diplostomum spathaceum, Diplodiscus subclavatus and Prosotocus confusus, were stained by novel benzanthrone dyes using different fixatives. The samples were examined under a confocal laser scanning microscope. All of the dyes tested demonstrated good results for digestive and reproductive system visualization. Based on obtained results we conclude that benzanthrone dyes could be used for internal and external structure confocal laser scanning microscopic imaging of trematode specimens.

Keywords

EN

benzanthrone dyes; trematode; confocal laser scanning microscopy

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 3
• Pages: 449-454

Dates

published

2018

received

2018-02-14

revised

2018-06-27

accepted

2018-07-12

(unknown)

2018-08-27

Contributors

author

Elena Kirilova

• Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia

author

Sanita Kecko

• Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia

author

Ligita Mežaraupe

• Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia

author

Inese Gavarāne

• Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia

author

Aleksandrs Pučkins

• Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia

author

Ilona Mickeviča

• Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia

author

Ilze Rubeniņa

• Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia

author

Sergejs Osipovs

• Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia

author

Andrejs Bulanovs

• Daugavpils University, Institute of Life Sciences and Technology, Department of Technology, Daugavpils, Latvia

author

Mihails Pupiņš

• Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia
• Latgales Zoo, Daugavpils, Latvia

author

Muza Kirjušina

• Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia

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Identifiers

DOI

10.18388/abp.2018_2574