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2018 | 65 | 3 | 449-454
Article title

Novel luminescent dyes for confocal laser scanning microscopy used in Trematoda parasite diagnostics

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EN
Abstracts
EN
Benzanthrone derivates are now widely used in many industrial and scientific applications as dyes for polymers and textiles. In biochemical, biomedical and diagnostics investigations benzanthrone dyes are used as a lipophilic fluorescent probe since many benzanthrone derivates demonstrate bright fluorescence and they have ability to intercalate between membrane lipids. The aim of research presented here was to assess the luminescence ability of benzanthrone derivatives using microscopic visualization of biological objects. Accordingly, specimens of freshwater trematodes: Diplostomum spathaceum, Diplodiscus subclavatus and Prosotocus confusus, were stained by novel benzanthrone dyes using different fixatives. The samples were examined under a confocal laser scanning microscope. All of the dyes tested demonstrated good results for digestive and reproductive system visualization. Based on obtained results we conclude that benzanthrone dyes could be used for internal and external structure confocal laser scanning microscopic imaging of trematode specimens.
Publisher

Year
Volume
65
Issue
3
Pages
449-454
Physical description
Dates
published
2018
received
2018-02-14
revised
2018-06-27
accepted
2018-07-12
(unknown)
2018-08-27
Contributors
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia
author
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Biotechnology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Technology, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia
  • Latgales Zoo, Daugavpils, Latvia
  • Daugavpils University, Institute of Life Sciences and Technology, Department of Ecology, Daugavpils, Latvia
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv65p449kz
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