Influence of oleic acid in different solvent media on BRL 3A cell growth and viability

• Authors: Runqi Liu , Wei Yang , Cheng Xia , Yuanyuan Chen , Sansi Gao , Zhihao Dong , Baoyin Huang , Ruirui Li , Ping He , Chuang Xu
• Languages of publication: EN

Abstracts

EN

Oleic acid (OA) is widely used in pathology studies of hepatocellular lipid deposition. Identifying the effects of different solvents on OA-induced liver lipid deposition would be beneficial for studies on hepatocytes. We treated BRL 3A cells with OA dissolved in different solvents. After 12 h incubation, cell viability was assessed using MTT assays. Reactive oxygen species (ROS), triglyceride (TG) and total cholesterol (TC) counts, and the expression level of glucose regulated protein (GRP78), sterol regulatory element binding protein (SREBP-1C) and fatty acid synthase (FAS) were analyzed. Water, PBS and DMSO were disadvantageous to the dissolution of OA and did not cause an OA-induced response in hepatocytes. In the alcohol+OA-treated cells, the severe ER stress, oxidative stress and cellular fat deposition were significantly increased. BSA promoted cell growth and the cells treated with 1.2% BSA+OA showed a lower grade TG and endoplasmic reticulum stress compared with KOH+OA and alcohol+OA treatments. KOH had no significant influence on BRL 3A cells viability. When treated with OA dissolved in KOH, BRL 3A cells showed a typical hepatocyte damage. KOH was considered the suitable choice for an OA solvent for BRL 3A cells in hepatic lipidosis research.

Keywords

EN

oleic acid; non-alcoholic fatty liver disease; liver lipid deposition

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 3
• Pages: 443-447

Dates

published

2018

received

2018-01-23

revised

2018-06-13

accepted

2018-06-19

(unknown)

2018-09-15

Contributors

author

Runqi Liu

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Wei Yang

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Cheng Xia

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Yuanyuan Chen

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Sansi Gao

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Zhihao Dong

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Baoyin Huang

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Ruirui Li

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Ping He

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

author

Chuang Xu

• College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China

References

• Caires KC, Shima CM, de Avila J, McLean DJ (2012) Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice. Reprod Toxicol 33: 76-84. doi: 10.1016/j.reprotox.2011.12.002.
• Cairns R, Alvarez-Guaita A, Martinez-Saludes I, Wason SJ, Hanh J, Nagarajan SR, Hosseini-Beheshti E, Monastyrskaya K, Hoy AJ, Buechler C, Enrich C, Rentero C, Grewal T (2017) Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation. Exp Cell Res doi: 10.1016/j.yexcr.2017.07.015.
• Chen L, Wang C, Huang S, Gong B, Yu J, Shi Q, Chen G (2014) Effects of individual and multiple fatty acids (palmitate oleate and docosahaexenoic acid) on cell viability and lipid metabolism in LO2 human liver cells. Mol Med Rep 10: 3254-3260. doi: 10.3892/mmr.2014.2579.
• Cinti DL, Cook L, Nagi MN, Suneja SK (1992) The fatty acid chain elongation system of mammalian endoplasmic reticulum. Prog Lipid Res 31: 1-51.
• Du X, Shi Z, Peng Z, Zhao C, Zhang Y, Wang Z, Li X, Liu G, Li X (2017) Acetoacetate induces hepatocytes apoptosis by the ROS-mediated MAPKs pathway in ketotic cows. J Cell Physiol 232: 3296-3308. doi: 10.1002/jcp.25773.
• Galvao J, Davis B, Tilley M, Normando E, Duchen MR, Cordeiro MF (2014) Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J 28: 1317-1330. doi: 10.1096/fj.13-235440.
• Imai K, Kudo N, Koyama M, Kawashima Y (2003) Effects of dehydroepiandrosterone on oleic acid accumulation in rat liver. Biochem Pharmacol 65: 1583-1591.
• Lagrutta LC, Montero-Villegas S, Layerenza JP, Sisti MS, Garcia de Bravo MM, Ves-Losada A (2017) Reversible nuclear-lipid-droplet morphology induced by oleic acid: a link to cellular-lipid metabolism. PLoS One 12: e0170608. doi: 10.1371/journal.pone.0170608.
• Li X, Huang W, Gu J, Du X, Lei L, Yuan X, Sun G, Wang Z, Li X, Liu G (2015) SREBP-1c overactivates ROS-mediated hepatic NF-kappaB inflammatory pathway in dairy cows with fatty liver. Cell Signal 27: 2099-2109. doi: 10.1016/j.cellsig.2015.07.011.
• Liao CC, Ou TT, Huang HP, Wang CJ (2014) The inhibition of oleic acid induced hepatic lipogenesis and the promotion of lipolysis by caffeic acid via up-regulation of AMP-activated kinase. J Sci Food Agric 94: 1154-1162. doi: 10.1002/jsfa.6386.
• Liao FH, Liou TH, Shieh MJ, Chien YW (2010) Effects of different ratios of monounsaturated and polyunsaturated fatty acids to saturated fatty acids on regulating body fat deposition in hamsters. Nutrition 26: 811-817. doi: 10.1016/j.nut.2009.09.009.
• Liu L, Li X, Li Y, Guan Y, Song Y, Yin L, Chen H, Lei L, Liu J, Li X, Wang Z, Yang X, Liu G (2014) Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro. J Dairy Sci 97: 1328-1335. doi: 10.3168/jds.2013-6654.
• Moravcova A, Cervinkova Z, Kucera O, Mezera V, Rychtrmoc D, Lotkova H (2015) The effect of oleic and palmitic acid on induction of steatosis and cytotoxicity on rat hepatocytes in primary culture. Physiol Res 64 (Suppl 5): S627-S636.
• Moslehi A, Nabavizadeh F, Zekri A, Amiri F (2017) Naltrexone changes the expression of lipid metabolism-related proteins in the endoplasmic reticulum stress induced hepatic steatosis in mice. Clin Exp Pharmacol Physiol 44: 207-212. doi: 10.1111/1440-1681.12695.
• Mota M, Banini BA, Cazanave SC, Sanyal AJ (2016) Molecular mechanisms of lipotoxicity and glucotoxicity in nonalcoholic fatty liver disease. Metabolism 65: 1049-1061. doi: 10.1016/j.metabol.2016.02.014.
• Muller C, Gardemann A, Keilhoff G, Peter D, Wiswedel I, Kropf S, Schild L (2010) Palmitate protects hepatocytes from oxidative stress and triacylglyceride accumulation by stimulation of nitric oxide synthesis in the presence of high glucose and insulin concentration. Free Radic Res 44: 1425-1434. doi: 10.3109/10715762.2010.512919.
• Muller C, Gardemann A, Keilhoff G, Peter D, Wiswedel I, Schild L (2012) Prevention of free fatty acid-induced lipid accumulation oxidative stress and cell death in primary hepatocyte cultures by a Gynostemma pentaphyllum extract. Phytomedicine 19: 395-401. doi: 10.1016/j.phymed.2011.12.002.
• Periasamy S, Chien SP, Chang PC, Hsu DZ, Liu MY (2014) Sesame oil mitigates nutritional steatohepatitis via attenuation of oxidative stress and inflammation: a tale of two-hit hypothesis. J Nutr Biochem 25: 232-240. doi: 10.1016/j.jnutbio.2013.10.013.
• Perla FM, Prelati M, Lavorato M, Visicchio D, Anania C (2017) The role of lipid and lipoprotein metabolism in non-alcoholic fatty liver disease. Children (Basel) 4: doi: 10.3390/children4060046.
• Rogue A, Antherieu S, Vluggens A, Umbdenstock T, Claude N, de la Moureyre-Spire C, Weaver RJ, Guillouzo A (2014) PPAR agonists reduce steatosis in oleic acid-overloaded HepaRG cells. Toxicol Appl Pharmacol 276: 73-81. doi: 10.1016/j.taap.2014.02.001.
• Seo MS, Hong SW, Yeon SH, Kim YM, Um KA, Kim JH, Kim HJ, Chang KC, Park SW (2014) Magnolia officinalis attenuates free fatty acid-induced lipogenesis via AMPK phosphorylation in hepatocytes. J Ethnopharmacol 157: 140-148. doi: 10.1016/j.jep.2014.09.031.
• Zhu S, Ma L, Wu Y, Zhang T, Zhang Q, Rasoul LM, Yunye MG, Zhou LB, Ren G, Li D (2014) FGF21 treatment ameliorates alcoholic fatty liver through activation of AMPK-SIRT1 pathway. Acta Biochim Biophys Sin (Shanghai) 46: 1041-1048. doi.org/10.1093/abbs/gmu097.
• Shi X, Li D, Deng Q, Li Y, Sun G, Yuan X, Song Y, Wang Z, Li X, Li X, Liu G (2015) NEFAs activate the oxidative stress-mediated NF-kappaB signaling pathway to induce inflammatory response in calf hepatocytes. J Steroid Biochem Mol Biol 145: 103-112. doi: 10.1016/j.jsbmb.2014.10.014.
• Song Y, Li N, Gu J, Fu S, Peng Z, Zhao C, Zhang Y, Li X, Wang Z, Li X, Liu G (2016) beta-Hydroxybutyrate induces bovine hepatocyte apoptosis via an ROS-p38 signaling pathway. J Dairy Sci 99: 9184-9198. doi: 10.3168/jds.2016-11219.
• Wang J, Zhu X, Wang Z, Yao J, Zhao B, Liu G (2015) Non-esterified fatty acids promote expression and secretion of angiopoietin-like protein 4 in calf hepatocytes cultured in vitro. Mol Cell Biochem 401: 141-146. doi: 10.1007/s11010-014-2301-2.
• Webster NJG (2017) Alternative RNA splicing in the pathogenesis of liver disease. Front Endocrinol (Lausanne) 8: 133. doi: 10.3389/fendo.2017.00133.
• Weng J, Li W, Jia X, An W (2017) Alleviation of ischemia-reperfusion injury in liver steatosis by augmenter of liver regeneration (ALR) is attributed to antioxidation and preservation of mitochondria. Transplantation. doi: 10.1097/TP.0000000000001874.
• Xu C, Sun LW, Xia C, Zhang HY, Zheng JS, Wang JS (2016a) (1)H-Nuclear magnetic resonance-based plasma metabolic profiling of dairy cows with fatty liver. Asian-Australas J Anim Sci 29: 219-229. doi: 10.5713/ajas.15.0439.
• Xu C, Xu Q, Chen Y, Yang W, Xia C, Yu H, Zhu K, Shen T, Zhang Z (2015) The relationship between Fibroblast Growth Factor-21 and characteristic parameters related to energy balance in dairy cows. BMC Vet Res 11: 271. doi: 10.1186/s12917-015-0585-4.
• Xu C, Xu Q, Chen Y, Yang W, Xia C, Yu H, Zhu K, Shen T, Zhang Z (2016b) FGF-21: promising biomarker for detecting ketosis in dairy cows. Vet Res Commun 40: 49-54. doi: 10.1007/s11259-015-9650-5.
• Zhang J, Li Y, Jiang S, Yu H, An W (2014) Enhanced endoplasmic reticulum SERCA activity by overexpression of hepatic stimulator substance gene prevents hepatic cells from ER stress-induced apoptosis. Am J Physiol Cell Physiol 306: C279-C290. doi: 10.1152/ajpcell.00117.2013.
• Zhang W, Shi Q, Wang X, Xie J, Li S, Wu Q (2004) Effects of different concentrations of DMSO on the growth of gastric carcinoma cells. Chongqing Medicine Magazine 33: 1731-1732.
• Zhao L, Ackerman SL (2006) Endoplasmic reticulum stress in health and disease. Curr Opin Cell Biol 18: 444-452. doi: 10.1016/j.ceb.2006.06.005.
• Zhou B, Zhou DL, Wei XH, Zhong RY, Xu J, Sun L (2017) Astragaloside IV attenuates free fatty acid-induced ER stress and lipid accumulation in hepatocytes via AMPK activation. Acta Pharmacol Sin 38: 998-1008. doi: 10.1038/aps.2016.175.

Identifiers

DOI

10.18388/abp.2018_2557