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2018 | 65 | 3 | 431-435
Article title

Overexpression of zeaxanthin epoxidase gene from Medicago sativa enhances the tolerance to low light in transgenic tobacco

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Zeaxanthin epoxidase (ZEP) plays an important role in xanthophyll cycle which is a process closely related to photosynthesis. However, an impact of ZEP on low-light stress has not been studied. In this study, the functions of an alfalfa (Medicago sativa) zeaxanthin epoxidase gene, MsZEP, in response to low-light stress were investigated by heterologous expression in tobacco (Nicotiana tabacum). Under normal light conditions, the measured parameters were not significantly different between transgenic and wild-type (WT) plants except for non-photochemical quenching value and chlorophyll a content. However, the differences were detected under low-light stress. We found that MsZEP-overexpression tobacco grew faster than WT (p≤0.05). The leaf fresh weight and leaf area of transgenic plants were significantly higher, and the number of stomata was greater in MsZEP-overexpression tobacco. As for photosynthetic characteristics, quantum yield of PSII (ΦPSII) and maximal photochemical efficiency of PSII (Fv/Fm) were not significantly different, whereas non-photochemical quenching (NPQ), net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of MsZEP-overexpression tobacco were significantly higher than in WT plants. However, no significant difference was detected between the two types of tobacco in chlorophyll and carotenoids content. In conclusion, MsZEP can improve the ability of tobacco to withstand low-light stress, which might be due to its stronger photosynthetic activity and the improvement of stomatal density under low light.
Physical description
  • Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  • College of Grassland Resources and Environment, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010000, China
  • Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  • Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  • Grassland Management Station of Shaanxi Province, Xi'an, Shaanxi, 710016, China
  • Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  • Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  • Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  • Agrawal GK, Yamazaki M, Kobayashi M, Hirochika R, Miyao A, Hirochika H (2001) Screening of the rice viviparous mutants generated by endogenous retrotransposon tos17 insertion. Tagging of a zeaxanthin epoxidase gene and a novel OsTATCGene. Plant Physiol 125: 1248-1257. doi: 10.1104/pp.125.3.1248.
  • Audran C, Borel C, Frey A, Sotta B, Meyer C, Simonneau T, Marion-Poll A (1998) Expression studies of the zeaxanthin epoxidase gene in Nicotiana plumbaginifolia. Plant Physiol 118: 1021-1028. doi: 10.1104/pp.118.3.1021.
  • Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL (2005) A mutational analysis of the ABA1 gene of Arabidopsis thaliana high lights the involvement of ABA in vegetative development. J Exp Bot 56: 2071-2083. doi: 10.1093/jxb/eri206.
  • Bell G, Danneberger T (1999) Temporal shade on creeping bentgrass turf. Crop Sci 39: 1142-1146. doi: 10.2135/cropsci1999.0011183X003900040032x
  • Bjorkman O, Boardman N, Anderson JM, Thorne S, Goodchild D, Pyliotis N (1972) Effect of light intensity during growth of Atriplex patula on the capacity of photosynthetic reactions, chloroplast components and structure. Carnegie Institution Year Book 71: 115-135.
  • Bugos RC, Hieber AD, Yamamoto HY (1998) Xanthophyll cycle enzymes are members of the lipocalin family, the first identified from plants. J Biol Chem 273: 15321-15324. doi: 10.1074/jbc.273.25.15321.
  • Della PD, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annu Rev Plant Biol 57: 711-738. doi: 10.1146/annurev.arplant.56.032604.144301.
  • Demmig-Adams B, Adams WW (1996) The role of xanthophyll cycle carotenoids in the protection of photosynthesis. Trends Plant Sci 1: 21-26. doi: 10.1016/S1360-1385(96)80019-7
  • Elhaddad NS, Hunt L, Sloan J, Gray JE (2014) Light-induced stomatal opening is affected by the guard cell protein kinase APK1b. PloS One 9: e97161. doi: 10.1371/journal.pone.0097161.
  • Farquhar GD, von Caemmerer S, Berry JA (2001) Models of photosynthesis. Plant Physiol 125: 42-45. doi: 10.1104/pp.125.1.42.
  • Franks PJ, Farquhar GD (2001) The effect of exogenous abscisic acid on stomatal development, stomatal mechanics, and leaf gas exchange in Tradescantia virginiana. Plant Physiol 125: 935-942. doi: 10.1104/pp.125.2.935.
  • Frommolt R, Goss R, Wilhelm C (2001) The de-epoxidase and epoxidase reactions of Mantoniella squamata (Prasinophyceae) exhibit different substrate-specific reaction kinetics compared to spinach. Planta 213: 446-456. doi: 10.1007/s004250100589.
  • Hieber AD, Bugos RC, Yamamoto HY (2000) Plant lipocalins: violaxanthin de-epoxidase and zeaxanthin epoxidase. Biochim Biophys Acta 1482: 84-91. doi: 10.1016/S0167-4838(00)00141-2.
  • Kang H, Zhu Y (2003) Observation of large Kerr nonlinearity at low light intensities. Phys Rev Lett 91: 093601. doi: 10.1103/PhysRevLett.91.093601.
  • Latowski D, Grzyb J, Strzałka K (2004) The xanthophyll cycle-molecular mechanism and physiological significance. Acta Physiol Plant 26: 197. doi: 10.1007/s11738-004-0009-8
  • Lee SH, Tewari RK, Hahn EJ, Paek KY (2007) Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania somnifera (L.) Dunal. plantlets. Plant Cell Tiss Org 90: 141-151. doi: 10.1016/j.cej.2004.01.016
  • Lichtenthaler H, Buschmann C, Döll M, Fietz HJ, Bach T, Kozel U, Meier D, Rahmsdorf U (1981) Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high-light and low-light plants and of sun and shade leaves. Photosynth Res 2: 115-141. doi: 10.1007/BF00028752.
  • Müller P, Li XP, Niyogi KK (2001) Non-photochemical quenching. A response to excess light energy. Plant Physiol 125: 1558-1566. doi: 10.1104/pp.125.4.1558.
  • Marin E, Nussaume L, Quesada A, Gonneau M, Sotta B, Hugueney P, Frey A, Marion PA (1996) Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana. EMBO J 15: 2331.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum 15: 473-497.
  • Murchie EH, Horton P (1997) Acclimation of photosynthesis to irradiance and spectral quality in British plant species: chlorophyll content, photosynthetic capacity and habitat preference. Plant Cell Environ 20: 438-448. doi: 10.1046/j.1365-3040.1997.d01-95.x
  • Nambara E, Marion-Poll A (2005) Abscisic acid biosynthesis and catabolism. Annu Rev Plant Biol 56: 165-185. doi: 10.1146/annurev.arplant.56.032604.144046.
  • Pan J, Guo B (2016) Effects of light intensity on the growth, photosynthetic characteristics, and flavonoid content of Epimedium pseudowushanense BL Guo. Molecules 21: E1475. doi: 10.3390/molecules21111475.
  • Park HY, Seok HY, Park BK, Kim SH, Goh CH, Lee BH, Lee CH, Moon YH (2008) Overexpression of Arabidopsis ZEP enhances tolerance to osmotic stress. Biochemical and biophysical research communications 375: 80-85. doi: 10.1016/j.bbrc.2008.07.128.
  • Pastenes C, Pimentel P, Lillo J (2005) Leaf movements and photoinhibition in relation to water stress in field-grown beans. J Exp Bot 56: 425-433. doi: 10.1093/jxb/eri061.
  • Peyami B, Mehmet E, Musa T, Ismet B (2008) Molecular and physiological changes in maize (Zea mays) induced by exogenous NAA, ABA and MeJa during cold stress. ANN BOT FENN 45: 173-185. doi: 10.5735/085.045.0302
  • Praba ML, Vanangamudi M, Thandapani V (2004) Effects of low light on yield and physiological attributes of rice. Int Rice Res Notes 29: 1-1.
  • Romanowska E, Powikrowska M, Zienkiewicz M, Drozak A, Pokorska B (2008) High light induced accumulation of two isoforms of the CF1 alpha-subunit in mesophyll and bundle sheath chloroplasts of C4 plants. Acta Biochim Pol 55: 175.
  • Sæbø A, Krekling T, Appelgren M (1995) Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tiss Org 41: 177-185. doi: 10.1007/bf00051588
  • Schwarz N, Armbruster U, Iven T, Brückle L, Melzer M, Feussner I, Jahns P (2014) Tissue-specific accumulation and regulation of zeaxanthin epoxidase in Arabidopsis reflect the multiple functions of the enzyme in plastids. Plant Cell Physiol 56: 346-357. doi: 10.1093/pcp/pcu167.
  • Singh V, Dey S, Murthy K (1988) Effect of low light stress on growth and yield of rice. Indian J Plant Physiol 31: 84-91.
  • Vasil'ev S, Wiebe S, Bruce D (1998) Non-photochemical quenching of chlorophyll fluorescence in photosynthesis. 5-hydroxy-1, 4-naphthoquinone in spinach thylakoids as a model for antenna based quenching mechanisms. BBA-Bioenergetics 1363: 147-156. doi: 10.1016/S0005-2728(97)00096-0.
  • Veres S, Tóth V, Láposi R, Oláh V, Lakatos G, Mészáros I (2006) Carotenoid composition and photochemical activity of four sandy grassland species. Photosynthetica 44: 255-261. doi: 10.1007/s11099-006-0016-7
  • Wang N, Fang W, Han H, Sui N, Li B, Meng QW (2008) Overexpression of zeaxanthin epoxidase gene enhances the sensitivity of tomato PSII photoinhibition to high light and chilling stress. Physiol Plant 132: 384-396. doi: 10.1111/j.1399-3054.2007.01016.x.
  • Wilson JW, Hand D, Hannah M (1992) Light interception and photosynthetic efficiency in some glasshouse crops. J Exp Bot 43: 363-373. doi: 10.1093/jxb/43.3.363
  • Xiong L, Lee H, Ishitani M, Zhu JK (2002) Regulation of osmotic stress-responsive gene expression by theLOS6/ABA1 locus in Arabidopsis. J Biol Chem 277: 8588-8596. doi: 10.1074/jbc.M109275200.
  • Yamamoto HY, Higashi R (1978) Violaxanthin de-epoxidase. Lipid composition and substrate specificity. Arch Biochem Biophys 190: 514-522. doi: 10.1016/0003-9861(78)90305-3.
  • Yang Y, Sun X, Yang S, Li X, Yang Y (2014) Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea. Biochem Biophys Res Commun 448: 145-150. doi: 10.1016/j.bbrc.2014.04.075.
  • Zhang Z, Wang Y, Chang L, Zhang T, An J, Liu Y, Cao Y, Zhao X, Sha X, Hu T (2016) MsZEP, a novel zeaxanthin epoxidase gene from alfalfa (Medicago sativa), confers drought and salt tolerance in transgenic tobacco. Plant Cell Rep 35: 439-453. doi: 10.1007/s00299-015-1895-5.
  • Zoratti L, Karppinen K, Luengo Escobar A, Häggman H, Jaakola L (2014) Light-controlled flavonoid biosynthesis in fruits. Front Plant Sci 5: 534. doi: 10.3389/fpls.2014.00534.
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