Transposon-associated polymorphisms of stress-responsive gene promoters in selected accessions of Arabidopsis thaliana

• Authors: Mladen Naydenov , Nadezhda Gospodinova , Elena Apostolova , Nikolay Anachkov , Vesselin Baev , Mariyana Gozmanova , Ivan Minkov , Galina Yahubyan
• Languages of publication: EN

Abstracts

EN

Genetic diversity caused by transposable element movement can play an important role in plant adaptation to local environments. Regarding genes, transposon-induced alleles were mostly related to gene bodies and a few of them to promoter regions. In this study, promoter regions of 9 stress-related genes were searched for transposable element insertions in 12 natural accessions of Arabidopsis thaliana. The promoter screening was performed via PCR amplification with primers designed to flank transposable element insertions in the promoter regions of the reference accession Col-0. Transposable element-associated insertion/deletion (indel) polymorphisms were identified in 7 of the 12 promoter loci across studied accessions that can be developed further as molecular markers. The transposable element absence in the promoter regions of orthologous genes in A. lyrata indicated that the insertion of these transposable elements in A. thaliana lineage had occurred after its divergence from A. lyrata. Sequence analysis of the promoter regions of CML41 (Calmodulin-like protein 41) and CHAP (chaperone protein dnaJ-related) confirmed the indel polymorphic sites in four accessions - Col-0, Wassilewskija, Shahdara, and Pirin. The observed indel polymorphism of the CHAP promoter region was associated with specific gene expression profiles in the different accessions grown at a normal and elevated temperature in a plant growth chamber. The collected data can be a starting point for gene expression profiling studies under conditions resembling the natural habitats of accessions.

Keywords

EN

transposable elements; promoters; natural accessions; Arabidopsis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 3
• Pages: 391-396

Dates

published

2018

received

2017-04-19

revised

2017-11-03

accepted

2018-05-21

(unknown)

2018-08-27

Contributors

author

Mladen Naydenov

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria

author

Nadezhda Gospodinova

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria

author

Elena Apostolova

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria

author

Nikolay Anachkov

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria

author

Vesselin Baev

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria
• Institute of Molecular Biology and Biotechnology, Plovdiv, Bulgaria

author

Mariyana Gozmanova

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria
• Institute of Molecular Biology and Biotechnology, Plovdiv, Bulgaria

author

Ivan Minkov

• Institute of Molecular Biology and Biotechnology, Plovdiv, Bulgaria

author

Galina Yahubyan

• Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria
• Institute of Molecular Biology and Biotechnology, Plovdiv, Bulgaria

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Identifiers

DOI

10.18388/abp.2017_1590