Acute hepatologic and nephrologic effects of calcitriol in Syrian golden hamster (Mesocricetus auratus)

• Authors: Ewa Podgorska , Martyna Sniegocka , Marianna Mycinska , Wojciech Trybus , Ewa Trybus , Anna Kopacz-Bednarska , Olga Wiechec , Martyna Krzykawska-Serda , Martyna Elas , Teodora Krol , Krystyna Urbanska , Andrzej Slominski
• Languages of publication: EN

Abstracts

EN

Although vitamin D is included in the group of fat-soluble vitamins, it must be considered as a prohormone. Its active forms, including calcitriol, have pleiotropic effects and play an important role in the regulation of cell proliferation, differentiation and apoptosis, as well as in hormone secretion, and they demonstrate anti-cancer properties. Since calcitriol delivery can be beneficial for the organism, and Syrian golden hamsters represent a unique experimental model, we decided to investigate its toxicity in this species. In this study, we injected calcitriol intraperitoneally at doses 0 (control), 0.180±0.009 µg/kg and 0.717±0.032 µg/kg. Animal behavior was observed for 72 hrs after injection, and afterwards blood, liver and kidneys were collected for post-mortem examination, electron microscopy, and hematology analyses. The highest dose of calcitriol induced a change in animal behavior from calm to aggressive, and the liver surface showed morphological signs of damage. Following injection of calcitriol, ultrastructural changes were also observed in the liver and kidneys, e.g. vacuolization and increased number of mitochondria. There was also a trend for increased serum levels of aspartate aminotransferase (AST), but not of alanine aminotransferase (ALT) or GGTP (gamma-glutamyl transpeptidase). There was no change in Ca, Mg and P levels, as well as in blood morphology between experimental and control groups. These results indicate that calcitriol at 0.717, but not at 0.180 µg/kg, may induce acute damage to the liver and kidneys, without inducing calcemia. We propose that the hepatotoxic effect of calcitriol in hamster constitutes the primary cause of behavioral changes.

Keywords

EN

calcitriol; Syrian golden hamster; hepatologic toxicity; nephrologic toxicity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 3
• Pages: 351-358

Dates

published

2018

received

2018-04-07

revised

2018-05-21

accepted

2018-06-06

(unknown)

2018-07-17

Contributors

author

Ewa Podgorska

• Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland

author

Martyna Sniegocka

• Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland

author

Marianna Mycinska

• Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland

author

Wojciech Trybus

• Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland

author

Ewa Trybus

• Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland

author

Anna Kopacz-Bednarska

• Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland

author

Olga Wiechec

• Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland

author

Martyna Krzykawska-Serda

• Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland

author

Martyna Elas

• Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland

author

Teodora Krol

• Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland

author

Krystyna Urbanska

• Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland

author

Andrzej Slominski

• Department of Dermatology, Comprehensive Cancer Center, Cancer Chemoprevention Program, University of Alabama at Birmingham, Birmingham, AL, USA
• VA Medical Center, Birmingham, AL, USA

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Identifiers

DOI

10.18388/abp.2018_2626