Peripheral regulatory T cells and anti-inflammatory cytokines in children with juvenile idiopathic arthritis

• Authors: Katarzyna Sznurkowska , Małgorzata Boćkowska , Maciej Zieliński , Katarzyna Plata-Nazar , Piotr Trzonkowski , Anna Liberek , Barbara Kamińska , Agnieszka Szlagatys-Sidorkiewicz
• Languages of publication: EN

Abstracts

EN

Background: Juvenile idiopathic arthritis (JIA) is a chronic, heterogenous inflammatory disease of unclear pathogenesis. JIA is hypothesized to be linked to a defective immune regulation. Anti-inflammatory cytokines belong to the best known regulatory factors. T-regulatory cells are a crucial cellular component of immune tolerance. One of their functions is synthesis of interleukin 10 (IL-10) and transforming growth factor beta1 (TGF-β1). The aim of this study was to determine the proportion of T-regulatory cells (CD4+CD25highFOXP3+) in peripheral blood, and serum levels of TGF-β1 and IL-10 in patients with JIA. Methods: The study included 25 patients with newly diagnosed JIA: oligoarthritis (n=17) and polyarthritis (n=8). The control group was comprised of 17 healthy children. CD4+CD25highFOXP3+ T cells in peripheral blood were quantified by means of three-color flow cytometry. Serum concentrations of TGF-β1 and IL-10 were estimated with ELISA. Results: The proportion of peripheral CD4+CD25highFOXP3+ cells in patients with JIA was significantly higher than in the controls (p=0.04). The two groups did not differ significantly in terms of their TGF-β1 and IL-10 concentrations. Conclusions: At the time of diagnosis, children with JIA presented with an elevated proportion of T-regulatory cells (CD4+CD25highFOXP3+) in peripheral blood. Anti-inflammatory cytokines, IL-10 and TGF-β1, are not upregulated in the serum of patients with JIA, and therefore should not be considered as biomarkers of this condition.

Keywords

EN

Tregs; IL-10; TGF-β1; juvenile idiopathic arthritis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 1
• Pages: 119-123

Dates

published

2018

received

2017-08-29

revised

2017-11-08

accepted

2017-11-09

(unknown)

2018-03-01

Contributors

author

Katarzyna Sznurkowska

• Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdańsk, Gdańsk, Poland

author

Małgorzata Boćkowska

• Department of Pediatric Rheumatology, Provincial Center of Rheumatology in Sopot, Sopot, Poland

author

Maciej Zieliński

• Department of Clinical Immunology and Transplantology, Medical University of Gdansk, Gdańsk, Poland

author

Katarzyna Plata-Nazar

• Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdańsk, Gdańsk, Poland

author

Piotr Trzonkowski

• Department of Clinical Immunology and Transplantology, Medical University of Gdansk, Gdańsk, Poland

author

Anna Liberek

• Faculty of Health Sciences with Subfaculty of Nursing, Medical University of Gdansk, Gdańsk, Poland

author

Barbara Kamińska

• Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdańsk, Gdańsk, Poland

author

Agnieszka Szlagatys-Sidorkiewicz

• Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdańsk, Gdańsk, Poland

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Identifiers

DOI

10.18388/abp.2017_2308