Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces

• Authors: Małgorzata Natonek-Wiśniewska , Piotr Krzyścin
• Languages of publication: EN

Abstracts

EN

The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A260/280 purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.

Keywords

EN

biological traces; forensic DNA analysis; species identification of forensic DNA; species identification of biological traces; mtDNA

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 4
• Pages: 705-708

Dates

published

2017

received

2017-08-17

revised

2017-11-17

accepted

2017-11-24

(unknown)

2017-12-13

Contributors

author

Małgorzata Natonek-Wiśniewska

• Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland

author

Piotr Krzyścin

• Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland

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Identifiers

DOI

10.18388/abp.2017_2304