Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells

• Authors: Héctor Sarabia-Sainz , Verónica Mata Haro , José Sarabia Sainz , Luz Vázquez-Moreno , Gabriela Montfort
• Languages of publication: EN

Abstracts

EN

Adhesion of enterotoxigenic (ETEC) E. coli to host intestinal cells is mediated by lectin-like fimbriae that bind to specific glycan moieties on the surfaces of enterocytes. To prevent in vitro binding of E. coli F4 fimbriae (F4 ETEC+) to piglet enterocytes, neoglycans were synthesized by the Maillard reaction conjugating lactose (Lac), galacto-oligosaccharides (GOS) or chitin oligosaccharides (Ochit) to porcine serum albumin (PSA). Neoglycans were characterized by SDS-PAGE, intrinsic tryptophan fluorescence and recognition by plant lectins, as well as by F4 ETEC variants. Electrophoretic patterns suggested the binding to PSA of 63, 13 and 2 molecules of Lac, GOS and Ochit, respectively. All neoglycans displayed quenching of tryptophan fluorescence consistent with the degree of glycation estimated by SDS-PAGE. Plant lectins recognized the neoglycans according to their specificity, whereas antigenic variants of F4 ETEC (ab, ac and ad) recognized PSA-Ochit and PSA-Lac with higher affinity than that for GOS. Neoglycans partially hindered the in vitro binding of F4+ ETEC to piglet enterocytes in a dose-dependent manner. The most effective blocking was observed with PSA-Lac that partially inhibited the adhesion of bacteria to enterocytes in a dose dependent manner, as quantified by flow cytometry. Increased production of the cytokines IL-6 and TNF-α was observed in response to F4+ ETEC infection of enterocytes and production was reduced in the presence of PSA-Ochit and PSA-GOS. These results suggest that neoglycans synthesized by the Maillard reaction could be useful in the prophylaxis of diarrhea in piglets.

Keywords

EN

Albumin glycation; biorecognition; anti-adhesion; E. coli

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 4
• Pages: 679-686

Dates

published

2017

received

2017-07-24

revised

2017-09-05

accepted

2017-09-06

(unknown)

2017-12-05

Contributors

author

Héctor Sarabia-Sainz

• Laboratorio de Bioquímica de Proteínas y Glicanos Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora 83304, México

author

Verónica Mata Haro

• Laboratorio de Bioquímica de Proteínas y Glicanos Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora 83304, México

author

José Sarabia Sainz

• Departamento de Investigación en Física, Universidad de Sonora, Hermosillo Sonora 83000, México

author

Luz Vázquez-Moreno

• Laboratorio de Bioquímica de Proteínas y Glicanos Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora 83304, México

author

Gabriela Montfort

• Laboratorio de Bioquímica de Proteínas y Glicanos Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora 83304, México

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Identifiers

DOI

10.18388/abp.2017_2199