Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods

• Authors: Urszula Nawrot , Beata Sulik-Tyszka , Ewelina Kurzyk , Martyna Mroczyńska , Katarzyna Włodarczyk , Marta Wróblewska , Grzegorz Basak , Anna Brillowska-Dąbrowska
• Languages of publication: EN

Abstracts

EN

The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified as Aspergillus fumigatus based on the DNA sequences of the ITS1-2 fragment of ribosomal DNA and the β-tubulin gene, out of them seventeen isolates showed wild-type cyp51A sequence and three were positive for the mutation TR34/L98H. All isolates were tested for the susceptibility to itraconazole (ITZ), voriconazole (VOR) and posaconasole (POS) using microdilution methods, according to EUCAST and CLSI protocols, as well as using Etest. The results of microdilution and Etests were analysed separately according to clinical breakpoints (CBP) defined by EUCAST version 7.0 and epidemiological cut off values (ECV). Etest as well as reference methods excellently recognised the WT isolates, which were susceptible to all tested triazoles, regardless of the method and CBP or ECV criteria used. The Etest recognized three non-WT isolates as resistant or intermediately sensitive to ITZ and POS and one as resistant to VOR. The categorical concordance between Etests and EUCAST and Etests and the CLSI method ranged from 90 to 100%. The interpretation of the results obtained from routine A. fumigatus Etests requires great caution. The use of the confirmative examinations with reference AST methods as well as with molecular tests is recommended.

Keywords

EN

Aspergillus fumigatus; triazole resistance; susceptibility testing; Etest; cyp51A sequence

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 4
• Pages: 631-634

Dates

published

2017

received

2017-03-23

revised

2017-06-02

accepted

2017-08-23

(unknown)

2017-11-30

Contributors

author

Urszula Nawrot

• Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wrocław Medical University, Wrocław, Poland

author

Beata Sulik-Tyszka

• Department of Microbiology, Central Clinical Hospital, Warsaw Medical University, Warsaw, Poland
• Department of Dental Microbiology, Warsaw Medical University, Warsaw, Poland

author

Ewelina Kurzyk

• Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

author

Martyna Mroczyńska

• Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

author

Katarzyna Włodarczyk

• Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wrocław Medical University, Wrocław, Poland

author

Marta Wróblewska

• Department of Microbiology, Central Clinical Hospital, Warsaw Medical University, Warsaw, Poland
• Department of Dental Microbiology, Warsaw Medical University, Warsaw, Poland

author

Grzegorz Basak

• Department of Haematology, Oncology and Internal Medicine, Medical University of Warsaw, Warsaw, Poland

author

Anna Brillowska-Dąbrowska

• Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

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Identifiers

DOI

10.18388/abp.2017_1571