Overexpression of ID1 reverses the repression of human dental pulp stem cells differentiation induced by TWIST1 silencing

• Authors: Izabela Maciejewska , Monika Sakowicz-Burkiewicz , Marta Krzeminska , Tadeusz Pawelczyk
• Languages of publication: EN

Abstracts

EN

Multiple studies showed that the cessation of TWIST1 expression is the prerequisite for osteoblasts' maturation. However, recent reports revealed that the function of TWIST1 is different in the dental pulp stem cells (DPSCs), where a high level of TWIST1 expression promoted DPSCs' differentiation. The aim of the study was to investigate the impact of TWIST1 and ID1 on the differentiation process in the human DPSCs. Methods: TWIST1 and ID1 expression in the DSPCs was modulated by lentivirus transduction. Genes expression was assessed with qRT-PCR. The proteins level was evaluated by Western blot. The DPSCs differentiation was assessed with the proliferation, alkaline phosphatase (ALP) activity, and calcium concentration assays. Results: TWIST1 silencing suppressed the expression of ID1 and both the early and late markers of odontoblasts' differentiation detected at the transcript and protein level. The forced overexpression of ID1 increased the expression of the late markers of odontoblasts differentiation but diminished the expression of the early markers. DPCSs with the silenced TWIST1 and subsequent ID1 overexpression displayed an increase in the expression of the late markers of odontoblasts differentiation. Cells with silenced TWIST1 and overexpressing ID1 had increased activity of ALP, higher calcium concentration and decreased proliferation rate. The high level of ID1 expression might be a critical factor stimulating DPSCs differentiation and it might compensate the repressed differentiation of DPSCs caused by TWIST1 silencing. Conclusion: The mutual correlation between the expression level of TWIST1 and ID1 might be a critical factor driving the process of the human odontoblasts' differentiation.

Keywords

EN

dental pulp stem cells; TWIST1; ID1; odontoblast differentiation; DSPP; DMP1

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 4
• Pages: 615-619

Dates

published

2017

received

2017-02-03

revised

2017-09-07

accepted

2017-10-02

(unknown)

2017-11-20

Contributors

author

Izabela Maciejewska

• Department of Dental Prosthodontics, Medical University of Gdansk, Gdańsk, Poland

author

Monika Sakowicz-Burkiewicz

• Department of Molecular Medicine, Medical University of Gdansk, Gdańsk, Poland

author

Marta Krzeminska

• Department of Dental Prosthodontics, Medical University of Gdansk, Gdańsk, Poland

author

Tadeusz Pawelczyk

• Department of Molecular Medicine, Medical University of Gdansk, Gdańsk, Poland

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Identifiers

DOI

10.18388/abp.2017_1522