PL EN


Preferences help
enabled [disable] Abstract
Number of results
2017 | 64 | 4 | 615-619
Article title

Overexpression of ID1 reverses the repression of human dental pulp stem cells differentiation induced by TWIST1 silencing

Content
Title variants
Languages of publication
EN
Abstracts
EN
Multiple studies showed that the cessation of TWIST1 expression is the prerequisite for osteoblasts' maturation. However, recent reports revealed that the function of TWIST1 is different in the dental pulp stem cells (DPSCs), where a high level of TWIST1 expression promoted DPSCs' differentiation. The aim of the study was to investigate the impact of TWIST1 and ID1 on the differentiation process in the human DPSCs. Methods: TWIST1 and ID1 expression in the DSPCs was modulated by lentivirus transduction. Genes expression was assessed with qRT-PCR. The proteins level was evaluated by Western blot. The DPSCs differentiation was assessed with the proliferation, alkaline phosphatase (ALP) activity, and calcium concentration assays. Results: TWIST1 silencing suppressed the expression of ID1 and both the early and late markers of odontoblasts' differentiation detected at the transcript and protein level. The forced overexpression of ID1 increased the expression of the late markers of odontoblasts differentiation but diminished the expression of the early markers. DPCSs with the silenced TWIST1 and subsequent ID1 overexpression displayed an increase in the expression of the late markers of odontoblasts differentiation. Cells with silenced TWIST1 and overexpressing ID1 had increased activity of ALP, higher calcium concentration and decreased proliferation rate. The high level of ID1 expression might be a critical factor stimulating DPSCs differentiation and it might compensate the repressed differentiation of DPSCs caused by TWIST1 silencing. Conclusion: The mutual correlation between the expression level of TWIST1 and ID1 might be a critical factor driving the process of the human odontoblasts' differentiation.
Publisher

Year
Volume
64
Issue
4
Pages
615-619
Physical description
Dates
published
2017
received
2017-02-03
revised
2017-09-07
accepted
2017-10-02
(unknown)
2017-11-20
Contributors
  • Department of Dental Prosthodontics, Medical University of Gdansk, Gdańsk, Poland
  • Department of Molecular Medicine, Medical University of Gdansk, Gdańsk, Poland
  • Department of Dental Prosthodontics, Medical University of Gdansk, Gdańsk, Poland
  • Department of Molecular Medicine, Medical University of Gdansk, Gdańsk, Poland
References
  • Batouli S, Miura M, Brahim J, Tsutsui TW, Fisher LW, Gronthos S et al. (2003) Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res 82: 976-981.
  • Bialek P, Kern B, Yang X, Schrock M, Sosic D, Hong N et al. (2004) A twist code determines the onset of osteoblast differentiation. Dev Cell 6: 423-435. doi: 10.1016/S1534-5807(04)00058-9.
  • Castanon I, Von SS, Kass J, Baylies MK (2001) Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development. Development 128: 3145-3159.
  • Connerney J, Andreeva V, Leshem Y, Mercado MA, Dowell K, Yang X et al. (2008) Twist1 homodimers enhance FGF responsiveness of the cranial sutures and promote suture closure. Dev Biol 318: 323-334. doi: 10.1016/j.ydbio.2008.03.037.
  • Connerney J, Andreeva V, Leshem Y, Muentener C, Mercado MA, Spicer DB (2006) Twist1 dimer selection regulates cranial suture patterning and fusion. Dev Dyn 235: 1345-1357. doi: 10.1002/dvdy.20717.
  • Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 97: 13625-13630. doi: 10.1073/pnas.240309797.
  • Hayashi M, Nimura K, Kashiwagi K, Harada T, Takaoka K, Kato H et al. (2007) Comparative roles of Twist-1 and Id1 in transcriptional regulation by BMP signaling. J Cell Sci 120: 1350-1357. doi: 10.1242/jcs.000067.
  • Ju W, Hoffmann A, Verschueren K, Tylzanowski P, Kaps C, Gross G, Huylebroeck D (2000) The bone morphogenetic protein 2 signaling mediator Smad1 participates predominantly in osteogenic and not in chondrogenic differentiation in mesenchymal progenitors C3H10T1/2. J Bone Miner Res 15: 1889-1899. doi: 10.1359/jbmr.2000.15.10.1889.
  • Laursen KB, Mielke E, Iannaccone P, Fuchtbauer EM (2007) Mechanism of transcriptional activation by the proto-oncogene Twist1. J Biol Chem 282: 34623-34633. doi: 10.1074/jbc.M707085200.
  • Li Y, Lu Y, Maciejewska I, Galler KM, Cavender A, D'Souza RN (2011) TWIST1 promotes the odontoblast-like differentiation of dental stem cells. Adv Dent Res 23: 280-284. doi: 10.1177/0022034511405387.
  • Maciejewska I, Sakowicz-Burkiewicz M, Pawelczyk T (2014) Id1 expression level determines the differentiation of human dental pulp stem cells. J Dent Res 93: 576-581. doi: 10.1177/0022034514530164.
  • Meng T, Huang Y, Wang S, Zhang H, Dechow PC, Wang X et al. (2015) Twist1 is essential for tooth morphogenesis and odontoblast differentiation. J Biol Chem 290: 29593-29602. doi: 10.1074/jbc.M115.680546.
  • Peng Y, Kang Q, Luo Q, Jiang W, Si W, Liu BA et al. (2004) Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells. J Biol Chem 279: 32941-32949. doi: 10.1074/jbc.M403344200.
  • Rice DP, Aberg T, Chan Y, Tang Z, Kettunen PJ, Pakarinen L et al. (2000) Integration of FGF and TWIST in calvarial bone and suture development. Development 127: 1845-1855.
  • Rice DP, Rice R, Thesleff I (2003) Molecular mechanisms in calvarial bone and suture development and their relation to craniosynostosis. Eur J Orthod 25: 139-148.
  • Rice R, Thesleff I, Rice DP (2005) Regulation of Twist, Snail, and Id1 is conserved between the developing murine palate and tooth. Dev Dyn 234: 28-35. doi: 10.1002/dvdy.20501.
  • Sakowicz-Burkiewicz M, Kitowska A, Grden M, Maciejewska I, Szutowicz A, Pawelczyk T (2013) Differential effect of adenosine receptors on growth of human colon cancer HCT 116 and HT-29 cell lines. Arch Biochem Biophys 533: 47-54. doi: 10.1016/j.abb.2013.02.007.
  • Song X, Liu S, Qu X, Hu Y, Zhang X, Wang T, Wei F (2011) BMP2 and VEGF promote angiogenesis but retard terminal differentiation of osteoblasts in bone regeneration by up-regulating Id1. Acta Biochim Biophys Sin (Shanghai) 43: 796-804. doi: 10.1093/abbs/gmr074.
  • Thisse C, Perrin-Schmitt F, Stoetzel C, Thisse B (1991) Sequence-specific transactivation of the Drosophila twist gene by the dorsal gene product. Cell 65: 1191-1201. doi: 10.1016/0092-8674(91)90014-P.
  • Zhang Y, Blackwell EL, McKnight MT, Knutsen GR, Vu WT, Ruest LB (2012) Specific inactivation of Twist1 in the mandibular arch neural crest cells affects the development of the ramus and reveals interactions with hand2. Dev Dyn 241: 924-940. doi: 10.1002/dvdy.23776.
  • Zhang Y, Hassan MQ, Li ZY, Stein JL, Lian JB, van Wijnen AJ, Stein GS (2008) Intricate gene regulatory networks of helix-loop-helix (HLH) proteins support regulation of bone-tissue related genes during osteoblast differentiation. J Cell Biochem 105: 487-496. doi: 10.1002/jcb.21844.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p615kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.