Flavanols from Japanese quince (Chaenomeles japonica) fruit suppress expression of cyclooxygenase-2, metalloproteinase-9, and nuclear factor-kappaB in human colon cancer cells

• Authors: Katarzyna Owczarek , Elżbieta Hrabec , Jakub Fichna , Dorota Sosnowska , Maria Koziołkiewicz , Jacek Szymański , Urszula Lewandowska
• Languages of publication: EN

Abstracts

EN

Natural polyphenols and polyphenol-rich extracts have been found to possess preventive and therapeutic potential against several types of cancers, including colorectal cancer (CRC), which is an example of an inflammation-associated cancer. This study examines the chemopreventive effect of a Japanese quince (Chaenomeles japonica) fruit flavanol preparation (JQFFP) on colon cancer SW-480 cells. JQFFP, rich in procyanidin monomers and oligomers, was found to inhibit the SW-480 cell viability by 40% at 150 µM catechin equivalents (CE) after 72 h incubation when compared to control, but it was non-toxic to normal colon fibroblast CCD-18Co cells. Furthermore, 100 µM CE JQFFP suppressed COX-2 mRNA expression to 36.7% of control values and protein expression to 77%. In addition, JQFFP reduced the MMP-9 protein expression (to 24% vs. control at 100 µM CE) and caused inhibition of its enzymatic activity (to 35% vs. control at 100 µM CE). Not only did JQFFP inhibit the COX-2 and MMP-9 levels, but it also reduced the NF-κB protein expression (to 65% of control) and phosphorylation of its p65 subunit (to 51%) at 100 µM CE. These results provide the first evidence that JQFFP inhibits COX-2, MMP-9, and NF-κB expression, suggesting that it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards the colon cancer SW-480 cells.

Keywords

EN

flavanols; Japanese quince (Chaenomeles japonica); colon cells; COX-2; MMP-9; NF-κB

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 3
• Pages: 567-576

Dates

published

2017

received

2017-04-26

revised

2017-07-05

accepted

2017-07-10

(unknown)

2017-08-09

Contributors

author

Katarzyna Owczarek

• Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland

author

Elżbieta Hrabec

• Department of Medical Enzymology, Faculty of Medicine, Medical University of Lodz, Łódź, Poland

author

Jakub Fichna

• Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland

author

Dorota Sosnowska

• Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland

author

Maria Koziołkiewicz

• Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland

author

Jacek Szymański

• Central Scientific Laboratory, Faculty of Health Sciences, Medical University of Lodz, Łódź, Poland

author

Urszula Lewandowska

• Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland

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Identifiers

DOI

10.18388/abp.2017_1599