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2017 | 64 | 3 | 567-576
Article title

Flavanols from Japanese quince (Chaenomeles japonica) fruit suppress expression of cyclooxygenase-2, metalloproteinase-9, and nuclear factor-kappaB in human colon cancer cells

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Abstracts
EN
Natural polyphenols and polyphenol-rich extracts have been found to possess preventive and therapeutic potential against several types of cancers, including colorectal cancer (CRC), which is an example of an inflammation-associated cancer. This study examines the chemopreventive effect of a Japanese quince (Chaenomeles japonica) fruit flavanol preparation (JQFFP) on colon cancer SW-480 cells. JQFFP, rich in procyanidin monomers and oligomers, was found to inhibit the SW-480 cell viability by 40% at 150 µM catechin equivalents (CE) after 72 h incubation when compared to control, but it was non-toxic to normal colon fibroblast CCD-18Co cells. Furthermore, 100 µM CE JQFFP suppressed COX-2 mRNA expression to 36.7% of control values and protein expression to 77%. In addition, JQFFP reduced the MMP-9 protein expression (to 24% vs. control at 100 µM CE) and caused inhibition of its enzymatic activity (to 35% vs. control at 100 µM CE). Not only did JQFFP inhibit the COX-2 and MMP-9 levels, but it also reduced the NF-κB protein expression (to 65% of control) and phosphorylation of its p65 subunit (to 51%) at 100 µM CE. These results provide the first evidence that JQFFP inhibits COX-2, MMP-9, and NF-κB expression, suggesting that it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards the colon cancer SW-480 cells.
Publisher

Year
Volume
64
Issue
3
Pages
567-576
Physical description
Dates
published
2017
received
2017-04-26
revised
2017-07-05
accepted
2017-07-10
(unknown)
2017-08-09
Contributors
  • Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland
  • Department of Medical Enzymology, Faculty of Medicine, Medical University of Lodz, Łódź, Poland
author
  • Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland
  • Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland
  • Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Łódź, Poland
  • Central Scientific Laboratory, Faculty of Health Sciences, Medical University of Lodz, Łódź, Poland
  • Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p567kz
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