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2017 | 64 | 3 | 551-559
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Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells

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The purpose of this study was to analyze the indolic, phenolic, and fatty acid content and antioxidant activity of garlic sprouts growing in the dark and in the daylight. The pro- or anti-inflammatory properties of the garlic sprout extract were investigated by evaluating the cyclooxygenase-2 (COX-2), prostaglandin E synthase (cPGES), glutathione S transferase (GSTM1), nuclear factor NF-κB, peroxisome proliferator-activated receptors (PPARs), and aryl hydrocarbon receptor (AhR) protein levels in the RAW 264.7 cells activated with lipopolysaccharide (LPS). The highest amount of total indolic (73.56 mg/100 g f.w.) and phenolic compounds (36.23 mg/100 g f.w.) was detected in garlic sprouts grown in the daylight. Studies on antioxidant activity (the FRAP and DPPH method) of garlic sprouts showed that this activity is significantly higher for sprouts grown in full access to light when compared to those grown in the dark. In garlic sprout extracts, α-linolenic acid (ALA) was found to be in greater amount. COX-2 and cPGES level was lower when compared to LPS alone activated cells. After garlic extract treatment, higher level of GSTM1, PPARΥ, cytosolic p50 and p65 protein, as well as a lower NF-ĸB p50/p65 activity was noted in the RAW 264.7 cells which suggested PPARs and AhR transrepression mechanism of NF-ĸB signalling. The obtained results indicate Allium sativum sprouts are a rich source of n-3 fatty acids, indolic and phenolic compounds characterized by anti-inflammatory and antioxidative activity, which may support their high therapeutic and dietary potential.
Physical description
  • Department of Radioligands, Jagiellonian University Medical College, Kraków, Poland
  • Department of Food Chemistry and Nutrition, Medical College, Jagiellonian University, Kraków, Poland
  • Department of Pharmaceutical Botany, Jagiellonian University Medical College, Kraków, Poland
  • Department of Pharmaceutical Botany, Jagiellonian University Medical College, Kraków, Poland
  • Department of Pharmaceutical Botany, Jagiellonian University Medical College, Kraków, Poland
  • Alvarez-Suarez JM, Giampieri F, González-Paramás AM, Damiani E, Astolfi P, Martinez-Sanchez G, Bompadre S, Quiles JL, Santos-Buelga C, Battino M (2012) Phenolics from monofloral honeys protect human erythrocyte membranes against oxidative damage. Food Chem Toxicol 50: 1508-1516. doi: 10.1016/j.fct.2012.01.042.
  • Anand R, Kaithwas G (2014) Anti-inflammatory potential of alpha-linolenic acid mediated through selective COX inhibition: computational and experimental data. Inflammation 37: 1297-1306. doi: 10.1007/s10753-014-9857-6.
  • Angiosperm Phylogeny Group (2009) An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Bot J Linn Soc 161: 105-121
  • Bat-Chen W, Golan T, Peri I, Ludmer Z, Schwartz B (2010) Allicin purified from fresh garlic cloves induces apoptosis in colon cancer cells via Nrf2. Nutr Cancer 62: 947-957. doi: 10.1080/01635581.2010.509837.
  • Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': the FRAP assay. Anal Biochem 239: 70-76. doi: 10.1006/abio.1996.0292.
  • Butkiewicz D, Grzybowska E, Phillips DH, Hemminki K, Chorazy M (2000) Polymorphisms of the GSTP1 and GSTM1 genes and PAH-DNA adducts in human mononuclear white blood cells. Environ Mol Mutagen 35: 99-105. doi: 10.1002/(SICI)1098-2280(2000)35:2<99::AID-EM4>3.0.CO;2-2.
  • Durak A, Ozturk HS, Olcay E, Güven C (2002) Effects of garlic extract supplementation on blood lipid and antioxidant parameters and atherosclerotic plaque formation process in cholesterol-fed rabbits. J Herb Pharmacother 2: 19-32. doi: 10.1300/j157v02n02_03.
  • Durak I, Kavutcu M, Aytac B (2004) Effects of garlic extract consumption on blood lipid and oxidant/antioxidant parameters in humans with high blood cholesterol. J Nutr Biochem 15: 373-377. doi: 10.1016/j.jnutbio.2004.01.005.
  • Edwards QT, Colquist S, Maradiegue A (2005) What's cooking with garlic: is this complementary and alternative medicine for hypertension? J Am Acad Nurse Pract 17: 381-385. doi: 10.1111/j.1745-7599.2005.00065.x.
  • Ellnain-Wojtaszek M, Zgórka G (1999) High-performance liquid chromatography and thin-layer chromatography of phenolic acids from Ginkgo biloba L. leaves collected within vegetative period. J Liquid Chromatog Related Technolog 22: 1459-1471. doi: 10.1081/JLC-100101744
  • Esposito E, Cuzzocrea S (2010) Antiinflammatory activity of melatonin in central nervous system. Curr Neuropharmacol 8: 228-242. doi: 10.2174/157015910792246155.
  • Gdula-Argasińska J, Garbacik A, Tyszka-Czochara M, Woźniakiewicz M, Paśko P, Czepiel J (2013) Identification of lipid derivatives in Hep G2 cells. Acta Biochim Pol 60: 811-815.
  • Gdula-Argasińska J, Czepiel J, Woźniakiewicz A, Wojtoń K, Grzywacz A, Woźniakiewicz M, Jurczyszyn A, Perucki W, Librowski T (2015a) n-3 fatty acids as resolvents of inflammation in the A549 cells. Pharmacol Rep 67: 610-615. doi: 10.1016/j.pharep.2015.01.001.
  • Gdula-Argasińska J, Czepiel J, Totoń-Żurańska J, Jurczyszyn A, Perucki W, Wołkow P (2015b) Docosahexaenoic acid regulates gene expression in HUVEC cells treated with polycyclic aromatic hydrocarbons. Toxicol Lett 236: 75-81. doi: 10.1016/j.toxlet.2015.05.002.
  • Gdula-Argasińska J, Czepiel J, Totoń-Żurańska J, Librowski T, Czapkiewicz A, Perucki W, Woźniakiewicz M, Woźniakiewicz A (2016a) n-3 Fatty acids regulate the inflammatory-state related genes in the lung epithelial cells exposed to polycyclic aromatic hydrocarbons. Pharmacol Rep 68: 319-328. doi: 10.1016/j.pharep.2015.09.001.
  • Gdula-Argasińska J, Bystrowska B (2016b) Docosahexaenoic acid attenuates in endocannabinoid synthesis in RAW 264.7 macrophages activated with benzo(a)pyrene and lipopolysaccharide. Toxicol Lett 258: 93-100. doi: 10.1016/j.toxlet.2016.06.017.
  • Graham J (2006) Purification of human erythrocyte 'ghosts'. In Cell Biology Protocols. Harris Jr, Graham J, Rick-Wood D eds, pp 137-138. John Wiley & Sons, LTD., Chichester.
  • Hubbard TD, Murray IA, Perdew GH (2015) Indole and tryptophan metabolism: endogenous and dietary routes to Ah receptor activation. Drug Metab Dispos 43: 1522-1535. doi: 10.1002/em.20474.
  • Iciek M, Kwiecień I, Włodek L (2009) Biological properties of garlic and garlic-derived organosulfur compounds. Environ Mol Mutagen 50: 247-265. doi: 10.1002/em.20474.
  • Kamanna VS, Chandrasekhara N (1980) Fatty acid composition of garlic (Allium sativum Linnaeus) lipids. JAOCS 57: 175-176. doi: 10.1007/BF02883781
  • Kaneez FS, Arshad SS (2007) The metabolism of serotonin in neuronal cells in culture and platelets. Exp Brain Res 183: 411-416. doi: 10.1007/s00221-007-1133-7.
  • Li QQ, Zhou SD, He XJ, Yu Y, Zhang YC, Wei XQ (2010) Phylogeny and biogeography of Allium (Amaryllidaceae: Allieae) based on nuclear ribosomal internal transcribed spacer and chloroplast rps16 sequences, focusing on the inclusion of species endemic to China. Ann Bot 106: 709-733. doi: 10.1093/aob/mcq177.
  • Muszyńska B, Łojewski M, Sułkowska-Ziaja K, Szewczyk A, Gdula-Argasińska J, Hałaszuk P (2016) In vitro cultures of Bacopa monnieri (L. Pennell) and an analysis of selected groups of biologically active metabolites in their biomass. Pharm Biol 54: 2443-2463. doi: 10.3109/13880209.2016.1158843.
  • Muszyńska B, Maślanka A, Sułkowska-Ziaja K, Opoka W, Szopa A (2014) Analysis of 5-methyltryptamine, L-tryptophan, 5-hydroxy-L-tryptophan and melatonin in the bulbs of Allium sativum by TLC methods coupled with densitometric detection. J Planar Chromatogr. - Mod TLC 27: 210–216. doi: 10.1556/jpc.27.2014.3.11
  • Muszyńska B, Sułkowska-Ziaja K (2012) Analysis of indole compounds in edible Basidiomycota species after thermal processing. Food Chem 132: 455-459. doi: 10.1016/j.foodchem.2011.11.021.
  • Muszyńska B, Sułkowska-Ziaja K, Ekiert H (2011) Indole compounds in fruiting bodies of some edible Basidiomycota species. Food Chem 125: 1306-1308. doi: 10.1016/j.foodchem.2010.10.056
  • Nagini S (2008) Cancer chemoprevention by garlic and its organosulfur compounds - panacea or promise? AntiCancer Agents Med Chem 8: 313–321. doi: 10.2174/187152008783961879.
  • Nagy ZS, Czimmerer Z, Szanto A, Nagy L (2013) Pro-inflammatory cytokines negatively regulate PPARγ mediated gene expression in both human and murine macrophages via multiple mechanisms. Immunobiology 218: 1336-1344. doi: 10.1016/j.imbio.2013.06.011.
  • Nguyen NT, Hanieh H, Nakahama T, Kishimoto T (2013) The roles of aryl hydrocarbon receptor in immune responses. Int Immunol 25: 335-343. doi: 10.1093/intimm/dxt011.
  • Nuutila AM, Puupponen-Pimia R, Aarni M, Oksman-Caldentey KM (2003) Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chem 81: 485-493. doi: 10.1016/s0308-8146(02)00476-4
  • Park SY, Seetharaman R, Ko MJ, Kim Y, Kim TH, Yoon MK, Kwak JH, Lee SJ, Bae YS, Choi YW (2014) Ethyl linoleate from garlic attenuates lipopolysaccharide-induced pro-inflammatory cytokine production by inducing heme oxygenase-1 in RAW264.7 cells. Int Immunopharmacol 19: 253-261. doi: 10.1016/j.intimp.2014.01.017.
  • Paśko P, Bartoń H, Zagrodzki P, Gorinstein S, Fołta M, Zachwieja Z (2009) Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth. Food Chem 115: 994-998. doi: 10.1016/j.foodchem.2009.01.037
  • Paśko P, Sułkowska-Ziaja K, Muszyńska B, Zagrodzki P (2014) Serotonin, melatonin, and certain indole derivatives profiles in rutabaga and kohlrabi seeds, sprouts, bulbs, and roots. LWT-Food Sci. Technology 59: 740-745. doi: 10.1016/j.lwt.2014.07.024
  • Paśko P, Gdula-Argasińska J, Podporska-Carroll J, Quilty B, Wietecha-Posłuszny R, Tyszka-Czochara M, Zagrodzki P (2015) Influence of selenium supplementation on fatty acids profile and biological activity of four edible amaranth sprouts as new kind of functional food. J Food Sci Technol 52: 4724-4736. doi: 10.1007/s13197-014-1602-5.
  • Queiroz YS, Ishimoto EY, Bastos DHM, Sampaio GM, Torres EAFS (2009) Garlic (Allium sativum L.) and ready-to-eat garlic products: in vitro antioxidant activity. Food Chem 115: 371-374. doi: 10.1016/j.foodchem.2008.11.105
  • Serhan CN (2015) Pro-resolving lipid mediators are leads for resolution physiology. Nature 510: 92-101. doi: 10.1038/nature13479.
  • Singer P, Shapiro H, Theilla M, Anbar R, Singer J, Cohen J (2008) Anti-inflammatory properties of omega-3 fatty acids in critical illness: novel mechanisms and an integrative perspective. Intensive Care Med 34: 1580-1592. doi: 10.1007/s00134-008-1142-4.
  • Singhal NK, Srivastava G, Agrawal S, Jain SK, Singh MP (2012) Melatonin as a neuroprotective agent in the rodent models of Parkinson's disease: is it all set to irrefutable clinical translation? Mol Neurobiol 45: 186-199. doi: 10.1007/s12035-011-8225-x.
  • Sovova M, Sova P (2004) Pharmaceutical importance of Allium sativum L. Hypolipemic effects in vitro and in vivo. Ceská a Slovenská farmacie 53: 117-123.
  • Stone TW, Mackay GM, Forrest CM, Clark CJ, Darlington LG (2003) Tryptophan metabolites and brain disorders. Clin Chem Lab Med 41: 852-859. doi: 10.1515/cclm.2003.129.
  • Tin Y (2009) Ah receptor and NF-ĸB interplay on the stage of epigenome. Biochem Pharmacol 77: 670-680. doi: 10.1016/j.bcp.2008.10.023.
  • Turner EH, Loftis JM, Blackwell AD (2006) Serotonin a la carte: Supplementation with the serotonin precursor 5-hydroxytryptophan. Pharmacol Ther 109: 325-338. doi: 10.1016/j.pharmthera.2005.06.004.
  • Wu KK, Cheng HH, Chang TC (2014) 5-methoxyindole metabolites of L-tryptophan: control of COX-2 expression, inflammation and tumorigenesis. J Biomed Sci 21: 17. doi: 10.1186/1423-0127-21-17.
  • Yang L, Yuan J, Liu L, Shi C, Wang L, Tian F, Liu F, Wang H, Shao C, Zhang Q, Chen Z, Qin W, Wen W (2013) α-Linolenic acid inhibits human renal cell carcinoma cell proliferation through PPAR-γ activation and COX-2 inhibition. Oncol Lett 6: 197-202. doi: 10.3892/ol.2013.1336.
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