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2017 | 64 | 3 | 543-549
Article title

Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis

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EN
Abstracts
EN
Vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. Most V. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. In the study presented here, a catalase from V. vulnificus (CAT-Vv) was purified to homogeneity after expression in Escherichia coli. The kinetics and function of CAT-Vv were examined. CAT-Vv catalyzed the reduction of H2O2 at an optimal pH of 7.5 and temperature of 35°C. The Vmax and Km values were 65.8±1.2 U/mg and 10.5±0.7 mM for H2O2, respectively. Mutational analysis suggests that amino acids involved in heme binding play a key role in the catalysis. Quantitative reverse transcription-PCR revealed that in V. vulnificus, transcription of CAT-Vv was upregulated by low salinity, heat, and oxidative stresses. This research gives new clues to help inhibit the growth of, and infection by V. vulnificus.
Year
Volume
64
Issue
3
Pages
543-549
Physical description
Dates
published
2017
received
2017-02-22
revised
2017-04-24
accepted
2017-04-24
(unknown)
2017-09-12
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p543kz
Identifiers
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