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2017 | 64 | 3 | 485-491
Article title

Assessment of liver antioxidant status and mitochondrial membrane composition of Plasmodium berghei-infected mice treated with selected antimalarials

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Abstracts
EN
The present study was aimed at determining and comparing the effects of Artecxin (ART), P - Alaxin (P-ALA), Lonart (LON) and Chloroquine (CQ) on oxidative stress parameters and mitochondrial membrane composition in the course of malaria infection. Six groups of five mice each categorized as healthy control (non-parasitized non-treated group), parasitized-non-treated (PnT), parasitized-chloroquine-treated (positive control), parasitized-Artecxin, -Lonart and -P-Alaxin-treated groups were used for the study. Hepatic antioxidant status was assessed with levels of malondialdehyde (MDA) and reduced glutathione (GSH) as well as activity of superoxide dismutase (SOD) and catalase (CAT) in the post mitochondrial and mitochondrial fractions. Mitochondrial membrane integrity was also evaluated with activity of succinate dehydrogenase and levels of phospholipids, cholesterol and proteins in the liver mitochondria. Results revealed that treatment of parasitized mice with the antimalarial drugs significantly (p<0.05) decreased hepatic malondialdehyde (MDA) and mitochondrial membrane phospholipids compared to parasitized untreated group. On the other hand, significantly (p<0.05) elevated succinate dehydrogenase (SDH) activity, mitochondrial membrane cholesterol level, GSH concentration, catalase (CAT) and superoxide dismutase (SOD) activity in the post mitochondrial fraction were obtained. Thus, antimalarial drugs distort mitochondrial membrane integrity and electron transfer but reduce the malaria-induced oxidative stress on the host.
Publisher

Year
Volume
64
Issue
3
Pages
485-491
Physical description
Dates
published
2017
received
2016-10-10
revised
2016-11-21
accepted
2016-11-24
(unknown)
2017-08-09
Contributors
author
  • Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-araba, P.M.B. 12003, Lagos, Nigeria
  • Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-araba, P.M.B. 12003, Lagos, Nigeria
References
  • Ackrell BAC, Johnson MK, Gunsalus RP, Cecchini G (1992) Structure and function of succinate dehydrogenase and fumarate reductase. In Chemistry and Biochemistry of Flavoproteins, Muller F ed, vol 3, pp 229-297, Boca Raton FL, CRC Press
  • Adisa RA, Sulaimon LA, Fabunmi A (2016) Toxicological and biochemical effects of therapeutic doses of Artecxin, Lonart and P-Alaxin in P. berghei-infected mice: A comparative study. Nig Qt J Hosp Med 26 (In press)
  • Akanbi OM, Odaibo AB, Afolabi KA, Ademowo OG (2009) Anti-MSP-1(19) antibody (IgG) and reactive oxygen species (ROS) response against malaria infection in pregnancy in South Western Nigeria. Asian Pac J Trop Med 2: 9-15. doi: 20093149485
  • Arinola AG, Onubogu DI, Salimonu LS (2005) Spleen weight, liver weight and levels of circulating immune complexes in vitamin deficient mice infected with Plasmodium berghei. Afr J Clin Exp Microb 6: 95-99. doi: AJCEM/2004008/2516
  • Atamna H, Ginsburg H (1993) Origin of reactive oxygen species in erythrocytes infected with Plasmodium falciparum. Mol Biochem Parasitol 61: 231-241. doi: 10.1016/0166-6851(93)90069-A.
  • Becker K, Tilley L, Vennerstrom JL, Roberts D, Rogerson S, Ginsburg H (2004) Oxidative stress in malaria parasite-infected erythrocytes: Host-parasite interactions. Int J Parasitol 34: 163-189. doi: doi: 10.1016/j.ijpara.2003.09.011.
  • Breman JG, Alilio MS, Mills A (2004) Conquering the intolerable burden of malaria: what's new, what's needed: a summary. Am J Trop Med Hyg 71 (Suppl 2): 1-15.
  • Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52: 302-310. doi: 10.1016/S0076-6879(78)52032-6.
  • Chen PS, Toribara TY, Warner H (1956) Micro determination of phosphorus. Analyt Chem 28: 1756-1758. doi: 10.1021/ac60119a033
  • Cheng Z, Ristow M (2013) Mitochondria and metabolic homeostasis. Antioxid Redox Signal 19: 240-242. doi: 10.1089/ars.2013.5255.
  • Chicco AJ, Sparagna GC (2007) Role of cardiolipin alterations in mitochondrial dysfunction and disease. Am J Physiol Cell Physiol 292: C33-C44. doi: 101152/ajpcell.00243.2006.
  • Egwunyenga AO, Isamah G, Nmorsi OP (2004) Lipid peroxidation and ascorbic acid levels in Nigeria children with acute falciparum malaria. Afri J Biotech 3: 560-563. doi: 10.5897/AJB2004.000-2110
  • Farombi EO, Shyntum YY, Emerole GO (2003) Influence of chloroquine treatment and P. falciparum malaria infection on some enzymatic and non-enzymatic anti-oxidant defense indices in humans. Drug Chem Toxicol 26: 59-71. doi: 10.1081/DCT-120017558.
  • Fred MS, Ghee TT (2013) Natural Products with antimalarial activity. Phytochemistry and Pharmacognosy http://www.eolss.net/Eolss-sampleAllChapter.aspx
  • Geller BL, Winge DR (1983) A method for distinguishing Cu, Zn-and Mn-containing superoxide dismutases. Anal Biochem 128: 86-92. doi: 10.1016/0003-2697(83)90348-2.
  • Gora D, Sandhya M, Shiv G, Praveen S (2006) Oxidative stress, α-tocopherol, ascorbic acid and reduced glutathione status in schizophrenics. Indian J Clin Biochem 21: 34-38. doi: 10.1016/j.tibs.2010.04.002.
  • Greenberg J, Nadel EM, Coatney GR (1954) Differences in survival of several inbred strains of mice and their hybrids infected with Plasmodium berghei. J Infect Dis 95: 114-116. http://www.jstor.org/stable/30100598.
  • Hunt NH, Stocker R (1990) Oxidative stress and the redox status of malaria infected erythrocytes. Blood Cells 16: 499-526.
  • Idonije OB, Festus O, Okhiai O, Akpamu, U (2011) Comparative study of the status of a biomarker of lipid peroxidation (Malondialdehyde) in patients with Plasmodium falciparum and Plasmodium vivax malaria infection. Asian J Biol Sci 4: 506-513. doi: 10.3923/ajbs.2011.506.513
  • Johnson D, Lardy HA (1967) Isolation of liver or kidney mitochondria. Methods Enzymol 10: 94-96. doi: 10.1016/0076-6879(67)10018-9
  • Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR (1974) Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11: 151-169. doi: 100.1159/000136482.
  • Joshi AS, Zhou J, Gohil VM, Chen S, Greenberg ML (2009) Cellular functions of cardiolipin in yeast. Biochim Biophys Acta 1793: 212-218. doi: 10.1016/j.bbamcr.2008.07.024.
  • King TE (1967) Preparation of succinate dehydrogenase and reconstitution of succinate oxidase. Methods Enzymol 10: 322-331. doi: 10.1016/0076-6879(67)10061-X
  • Krishna S, Uhlemann A, Haynes RK (2004) Artemisinins: mechanisms of action and potential for resistance. Drug Resist Updat 7: 233-244. doi: 10.1016/j.drup.2004.07.001.
  • Kroemer G, Galluzzi L, Brenner C (2007) Mitochondrial membrane permeabilization in cell death. Physiol Rev 87: 99-163. doi: 10.1152/physrev.00013.2006.
  • Krungkrai SR, Yuthavong Y (1987) The antimalarial action on Plasmodium falciparum of qinghaosu and artesunate in combination with agents which modulate oxidant stress. Trans Roy Soc Trop Med Hyg 81: 710-714. doi: 10.1016/0035-9203(87)90003-4.
  • Kulkarni AG, Suryakar AN, Sardeshmukh AS, Rathi DB (2003) Studies on biochemical changes with special reference to oxidant and antioxidants in malaria patients. Indian J Clin Biochem 18: 136-149. doi: 10.1007/BF02867380.
  • Lancaster CR, Herzog E, Juhnke HD, Madej MG, Müller FG, Paul R, Schleidt PG (2008) Electroneutral and electrogenic catalysis by dihaem-containing succinate:quinone oxidoreductases. Biochem Soc Trans 36: 996-1000. doi: org/10.1042/BST0360996.
  • Levander OA, Ager AL Jr, Morris VC, May RG (1989) Qinghaosu, dietary vitamin E, selenium, and cod-liver oil: effect on the susceptibility of mice to the malarial parasite Plasmodium yoelli. Am J Clin Nutr 50: 346-352.
  • Lennon SV, Martin SJ, Cotter TG (1991) Dose-dependent induction of apoptosis in human cell lines by widely diverging stimuli. Cell Prolif 24: 203-214. doi: 10.1111/j.1365-2184.1991.tb01150.x.
  • Loria P, Miller S, Foley M, Tilley L (1999) Inhibition of the peroxidative degradation of heme as the basis of action of chloroquine and other quinoline anti-malarials. Biochem J 339: 363-370. doi: 10.1042/bj3390363.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
  • Malhi H, Guicciardi ME, Gores GJ (2010) Hepatocyte death: a clear and present danger. Physiol Rev 90: 1165-1194. doi: 10.1152/physrev.00061.2009.
  • Meshnick SR, Tsang TW, Lin FB, Pan HZ, Chang CN, Kuypers F, Chiu D, Lubin B (1989) Activated oxygen mediates the antimalarial activity of qinghaosu. Prog Clin Biol Res 313: 95-104.
  • Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247: 3170-3175.
  • Nohl H, Gille L, Staniek K (2005) Intracellular generation of reactive oxygen species by mitochondria. Biochem Pharmacol 69: 719-723. doi: 10.1016/j.bcp.2004.12.002.
  • Ohtsuka T, Nishijima M, Suzuki K, Akamatsu YY (1993) Mitochondrial dysfunction of a cultured chinese hamster ovary cell mutant defient in cardiolipin. J Biol Chem 268: 22914-22919.
  • Olayemi SO, Arikawe AP, Akinyede A, Oreagba AI, Awodele O (2012) Effect of malarial treatments on biochemical parameters and plasma pH of mice infected with Plasmodium berghei. Int J Pharmacol 8: 549-554. doi: 10.3923/ijp.2012.549.554
  • Onyesom I, Agho JE (2011) Changes in serum glucose and triacylglycerol levels induced by the co-administration of two different types of antimalarial drugs among some Plasmodium falciparum malarial patients in Edo - Delta region of Nigeria. Asian J Sci Res 4: 78–83. doi: 10.3923/ajsr.2011.78.83
  • Orrenius S, Gogvadze V, Zhivotovsky B (2007) Mitochondrial oxidative stress: implications for cell death. Annu Rev Pharmacol Toxicol 47: 143-183. doi: 10.1146/annurev.pharmtox.47.120505.105122.
  • Pennacchio LA (2003) Insights from human/mouse genome comparisons. Mamm Genome 14: 429-436. doi: 10.1007/s00335-002-4001-1.
  • Reis PA, Comim CM, Hermani F, Silva B, Barichello T, Portella AC, Gomes FC, Sab IM, Frutuoso VS, Oliveira MF, Bozza PT, Bozza FA, Dal-Pizzol F, Zimmerman GA, Quevedo J, Castro-Faria-Neto HC (2010) Cognitive dysfunction is sustained after rescue therapy in experimental cerebral malaria, and is reduced by additive antioxidant therapy. PLoS Pathogens 6: e1000963. doi: 10.1371/journal.ppat.1000963.
  • Ribas V, Garcia-Ruiz C, Fernandez-Checa JC (2014) Glutathione and mitochondria. Front Pharmacol 5: 151. doi: 10.3389/fphar.2014.00151.
  • Rustin P, Munnich A, Rötig A (2002) Succinate dehydrogenase and human diseases: new insights into a well-known enzyme. Eur J Hum Genet 10: 289-291. doi: 10.1038/sj.ejhg.5200793.
  • Scott MP, Tamkun JW, Hartzell GW (1989) The structure and function of the homeodomain. Biochim Biophys Acta 989: 25-48. doi: 10.1016/0304-419X(89)90033-4.
  • Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and non protein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 25: 192-205. doi: 10.1016/0003-2697(68)90092-4.
  • Senok AC, Li K, Nelson EA, Yu LM, Tian LP, Oppenheimer SJ (1997) Invasion and growth of Plasmodium falciparum is inhibited in fractionated thalassaemic erythrocytes. Trans R Soc Trop Med Hyg 91: 138-143. doi: 10.1016/S0035-9203(97)90200-5.
  • Sinha KA (1972) Colorimetric assay of catalase. Anal Biochem 47: 389-394. doi: 10.1016/0003-2697(72)90132-7.
  • Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI (2005) The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature 434: 214-217. doi: 10.1038/nature03342.
  • Sowunmi A (1996) Hepatomegaly in acute falciparum malaria in children. Trans R Soc Trop Med Hyg 90: 540-542. doi: 10.1016/S0035-9203(96)90313-2.
  • Vennerstrom JL, Nuzum EO, Miller RE, Dorn A, Gerena L, Dande PA, Ellis WY, Ridley RG, Milhous WK (1999) 8-Aminoquinolines active against blood stage Plasmodium falciparum in vitro inhibit hematin polymerization. Antimicrob Agents Chemother 43: 598-602. doi: 0066-4804/99/$04.00+0.
  • Wei N, Sadrzadeh SM (1994) Enhancement of hemin-induced membrane damage by Artemisinin. Biochem Pharmacol 48: 737-741. doi: 10.1016/0006-2952(94)90051-5.
  • Yoshida S, Nagumo H, Yokomine T, Araki H, Suzuki A, Matsuoka H (2010) Plasmodium berghei circumvents immune responses induced by merozoite surface protein 1- and apical membrane antigen 1- based vaccines. PLoS One 5: e13727. doi: 10.1371/journal.pone.0013727.
  • Young TA, Cunningham CC, Bailey SM (2002) Reactive oxygen species production by the mitochondrial respiratory chain in isolated rat hepatocytes and liver mitochondria: studies using myxothiazol. Arch Biochem Biophys 405: 65-72. doi: 10.1016/S0003-9861(02)00338-7.
  • Zinser E, Sperka-Gottlieb CD, Fasch EV, Kohlwein SD, Paltauf F, Daum G (1991) Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae. J Bacteriol 173: 2026-2034. doi: 10.1128/jb.173.6.2026-2034.1991.
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Publication order reference
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bwmeta1.element.bwnjournal-article-abpv64p485kz
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