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2017 | 64 | 3 | 477-483
Article title

Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata

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Abstracts
EN
In this study, a 39-kDa metalloprotease was purified from a rare edible mushroom with health-promoting activities, Oudemansiella radicata, using a purification protocol which entailed anion exchange chromatography on DEAE-cellulose and Q-Sepharose columns and gel filtration by fast protein liquid chromatography on a Superdex 75 column. Some peptide sequences were obtained by LC-MS/MS analysis and one of the sequences, DAWIQADVNR, manifested 90% identity to Coprinopsis cinerea metalloprotease. The optimal reaction pH and temperature for Oudemansiella radicata protease were pH 7.0 and 50°C, respectively. The protease was purified 79-fold and demonstrated a specific protease activity of 2.42 U/mg. The Km of the purified protease for the casein substrate was 0.65 mg/mL at pH 7.0 and 50°C. The activity of the protease was inhibited by Cd2+, Hg2+, Cu2+, Pb2+ and Fe3+ ions, but was enhanced by K+, Mn2+ and Fe2+ ions. The marked suppression of the protease activity by EDTA indicates that the protease is a metalloprotease.
Publisher

Year
Volume
64
Issue
3
Pages
477-483
Physical description
Dates
published
2017
received
2016-09-26
revised
2017-03-10
accepted
2017-05-08
(unknown)
2017-09-08
Contributors
author
  • College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China
  • State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
author
  • State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
author
  • Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China
  • Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China
author
  • College of Food Science and Technology, Nanjing Agricultural University, Weigang, Nanjing 210095, China
author
  • State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
author
  • School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p477kz
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