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2017 | 64 | 3 | 471-475
Article title

Helicobacter pylori cytotoxin-associated gene A impairs the filtration barrier function of podocytes via p38 MAPK signaling pathway

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EN
Abstracts
EN
Helicobacter pylori (Hp) specific antigens were found deposited in the glomeruli in some kidney diseases. However, the underlying molecular mechanisms remain to be elucidated. The aim of this study was to investigate the effect of cytotoxin associated gene A protein (CagA), a key virulence factor of Hp, on mouse podocytes. Cells were cultured and treated with recombinant CagA protein. The expression of the tight junction protein ZO-1 and p38 MAPK signaling pathway activation were measured with real-time RT-PCR and western blotting. The filtration barrier function of podocytes was evaluated with albumin influx assay. CagA decreased the expression and membrane distribution of ZO-1, impaired the filtration barrier function of podocytes, while activating p38 MAPK signaling pathway in these cells. Selective p38 MAPK inhibition partly prevented CagA-induced filtration barrier dysfunction of podocytes through ameliorating ZO-1 downregulation. Taken together, the results suggested that CagA, at least via p38 MAPK signaling pathway, may induce podocyte injury. Anti-Hp therapy may be beneficial for the treatment of kidney diseases related to Hp antigen deposition.
Keywords
EN
Publisher

Year
Volume
64
Issue
3
Pages
471-475
Physical description
Dates
published
2017
received
2016-05-22
revised
2016-08-15
accepted
2016-08-17
(unknown)
2017-08-12
Contributors
author
  • Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
author
  • Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
author
  • Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
author
  • Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p471kz
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