Preliminary report on β-casein gene Met183QVal183 polymorphism in Romanian indigenous Zackel sheep breeds

• Authors: Szilvia Kusza , Daniela Ilie , Maria Sauer , Ioan-Walter Sauer , Dinu Gavojdian
• Languages of publication: EN

Abstracts

EN

Genetic polymorphisms of the milk protein genes are important because of their effects on quantitative traits and technological properties of milk manufacturing. In the present study we identified the polymorphism of the beta-casein gene in two local sheep breeds (Racka n=98 and Turcana n=111) in Romania. The most studied variants at the ovine beta-casein (CSN2) locus are: A and G variants. Genomic DNA was extracted from hair follicles and beta-casein genotypes were determined by the rapid TaqMan (Applied Biosystems, USA) genotyping assay. Homozygote genotypes GG were not detected in any of the studied breeds. In both, the Racka and Turcana breeds, the A variant had a much higher frequency, 0.98% and 0.97%, respectively. In the current study, the fast DNA tests for genotyping ovine CSN2 were successfully optimized, however, further samples and correlations of genomic results with milk characteristics and production data are needed for the development of future selection schemes of the Romanian indigenous sheep breeds, with the ultimate purpose to produce low allergen level sheep milk and derived dairy products.

Keywords

EN

β-casein; gene polymorphism; ovine milk; Racka; Turcana

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 339-341

Dates

published

2017

received

2016-10-05

revised

2017-02-21

accepted

2017-02-28

(unknown)

2017-05-17

Contributors

author

Szilvia Kusza

• Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, 325400, Caransebes, Romania
• Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary

author

Daniela Ilie

• Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, 325400, Caransebes, Romania
• Research and Development Station for Bovine Arad, Academy for Agricultural and Forestry Sciences, 317236, Arad, Romania

author

Maria Sauer

• Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, 325400, Caransebes, Romania

author

Ioan-Walter Sauer

• Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, 325400, Caransebes, Romania

author

Dinu Gavojdian

• Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, 325400, Caransebes, Romania

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Identifiers

DOI

10.18388/abp.2016_1437