MMP-10, MMP-7, TIMP-1 and TIMP-2 mRNA expression in esophageal cancer

• Authors: Agnieszka Juchniewicz , Oksana Kowalczuk , Robert Milewski , Wojciech Laudański , Piotr Dzięgielewski , Mirosław Kozłowski , Jacek Nikliński
• Languages of publication: EN

Abstracts

EN

Introduction: Tissue inhibitors of metalloproteinases (TIMP) and the matrix metalloproteinases (MMP) are involved in the spread of cancer. Methods: We have evaluated the matrix metalloproteinases' (MMP-10, MMP-7) and their inhibitors' (tissue inhibitors of metalloproteinases - TIMP-1, TIMP-2) mRNA expression in 61 esophageal cancer samples from patients who had undergone surgery, by using real-time quantitative RT-PCR, and correlated the results with the patient clinicopathologic features. Results: MMP-10, MMP-7, TIMP-1, TIMP-2 were overexpressed in 73%, 85%, 55% and 42% of esophageal cancer samples, respectively. The expression of MMP-10, TIMP-1, and TIMP-2 correlated with the tumor size. The MMP-7 overexpression was associated with the tumour stage (I, II vs III, p=0.05) and lymph node metastasis (N0 vs N1, p=0.037). Conclusions: We conclude that in the resected esophageal cancer an increased mRNA expression of MMP-7, MMP-10 and TIMP-1 correlated with clinicopathologic features. We suggest that these genes may play a role during progression of the disease.

Keywords

EN

Esophageal cancer; gene expression; metalloproteinases

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 295-299

Dates

published

2017

received

2016-08-16

revised

2017-01-12

accepted

2017-02-01

(unknown)

2017-05-15

Contributors

author

Agnieszka Juchniewicz

• Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland

author

Oksana Kowalczuk

• Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland

author

Robert Milewski

• Department of Statistic and Medical Informatics, Medical University of Bialystok, Bialystok, Poland

author

Wojciech Laudański

• Department of Thoracic Surgery, Medical University of Bialystok, Bialystok, Poland

author

Piotr Dzięgielewski

• Department of Thoracic Surgery, Medical University of Bialystok, Bialystok, Poland

author

Mirosław Kozłowski

• Department of Thoracic Surgery, Medical University of Bialystok, Bialystok, Poland

author

Jacek Nikliński

• Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland
• Department of Thoracic Surgery, Medical University of Bialystok, Bialystok, Poland

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Identifiers

DOI

10.18388/abp.2016_1408