Expression of the insect metalloproteinase inhibitor IMPI in the fat body of Galleria mellonella exposed to infection with Beauveria bassiana

• Authors: Lidiia Vertyporokh , Iwona Wojda
• Languages of publication: EN

Abstracts

EN

The inducible metalloproteinase inhibitor (IMPI) discovered in Galleria mellonella is currently the only specific inhibitor of metalloproteinases found in animals. Its role is to inhibit the activity of metalloproteinases secreted by pathogenic organisms as virulence factors to degrade immune-relevant polypeptides of the infected host. This is a good example of an evolutionary arms race between the insect hosts and their natural pathogens. In this report, we analyze the expression of a gene encoding an inducible metalloproteinase inhibitor (IMPI) in fat bodies of the greater wax moth larvae Galleria mellonella infected with an entomopathogenic fungus Beauveria bassiana. We have used a natural infection, i.e. covering larval integument with fungal aerospores, as well as injection of fungal blastospores directly into the larval hemocel. We compare the expression of IMPI with the expression of genes encoding proteins with fungicidal activity, gallerimycin and galiomycin, whose expression reflects the stimulation of Galleria mellonella defense mechanisms. Also, gene expression is analyzed in the light of survival of animals after spore injection.

Keywords

EN

Insect immunity; Galleria mellonella; Beauveria bassiana; IMPI

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 273-278

Dates

published

2017

received

2016-06-17

revised

2016-10-12

accepted

2016-10-14

(unknown)

2017-04-11

Contributors

author

Lidiia Vertyporokh

• Maria Curie-Sklodowska University, Faculty of Biology and Biotechnology, Institute of Biology and Biochemistry, Department of Immunobiology, Lublin, Poland

author

Iwona Wojda

• Maria Curie-Sklodowska University, Faculty of Biology and Biotechnology, Institute of Biology and Biochemistry, Department of Immunobiology, Lublin, Poland

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Identifiers

DOI

10.18388/abp.2016_1376