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2017 | 64 | 2 | 273-278
Article title

Expression of the insect metalloproteinase inhibitor IMPI in the fat body of Galleria mellonella exposed to infection with Beauveria bassiana

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EN
Abstracts
EN
The inducible metalloproteinase inhibitor (IMPI) discovered in Galleria mellonella is currently the only specific inhibitor of metalloproteinases found in animals. Its role is to inhibit the activity of metalloproteinases secreted by pathogenic organisms as virulence factors to degrade immune-relevant polypeptides of the infected host. This is a good example of an evolutionary arms race between the insect hosts and their natural pathogens. In this report, we analyze the expression of a gene encoding an inducible metalloproteinase inhibitor (IMPI) in fat bodies of the greater wax moth larvae Galleria mellonella infected with an entomopathogenic fungus Beauveria bassiana. We have used a natural infection, i.e. covering larval integument with fungal aerospores, as well as injection of fungal blastospores directly into the larval hemocel. We compare the expression of IMPI with the expression of genes encoding proteins with fungicidal activity, gallerimycin and galiomycin, whose expression reflects the stimulation of Galleria mellonella defense mechanisms. Also, gene expression is analyzed in the light of survival of animals after spore injection.
Publisher

Year
Volume
64
Issue
2
Pages
273-278
Physical description
Dates
published
2017
received
2016-06-17
revised
2016-10-12
accepted
2016-10-14
(unknown)
2017-04-11
Contributors
  • Maria Curie-Sklodowska University, Faculty of Biology and Biotechnology, Institute of Biology and Biochemistry, Department of Immunobiology, Lublin, Poland
author
  • Maria Curie-Sklodowska University, Faculty of Biology and Biotechnology, Institute of Biology and Biochemistry, Department of Immunobiology, Lublin, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p273kz
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