Analysis of glycosylated flavonoids extracted from sweet-cherry stems, as antibacterial agents against pathogenic Escherichia coli isolates

• Authors: Alfredo Aires , Carla Dias , Rosa Carvalho , Maria Saavedra
• Languages of publication: EN

Abstracts

EN

The aim of this study was to evaluate the bioactivity of flavonoids extracted from sweet-cherry stems which are often used by a traditional system of medicine to treat gastro-intestinal and urinary tract infections but lacking any consistent scientific evidence; moreover the information about the class of phenolics, their content and the potential bioactivity of such material is very scarce. Thus, in this context, we have set a research study in which we evaluated the profile and content of phenolics extracted from sweet-cherry stems through a conventional (70ºC and 20 min) and ultrasound assisted extraction (40 kHz, room temperature and 20 min). The extracts were phytochemically characterized by using an HPLC-DAD-UV/VIS system and assayed by an in vitro minimum inhibitory concentration (MIC) bioassay against Escherichia coli isolates. Simultaneously, the total antioxidant activities were measured using the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS•+) radical cation assay. Our results indicate that sweet-cherry stems have a high content of sakuranetin, ferulic acid, p-coumaric acid, p-coumaroylquinic acid, chlorogenic acid and its isomer neochlorogenic acid. Their average levels were highly affected by the extraction method used (p<0.001). The same trend was observed for total antioxidant activity and MIC values. The extracts produced with ultrasounds presented both, a higher total antioxidant activity and a lower minimum inhibitory concentration. Statistical analyses of our results showed a significant correlation (p<0.01) of total antioxidant activity and minimum inhibitory concentration with phenolics present in the extracts studied. Thus, we can conclude that cherry stems can be further exploited to purify compounds and produce coproducts with enhanced biologically added value for pharmaceutical industry.

Keywords

EN

flavonoids; agro-food wastes; enhanced extraction; antimicrobial agents; pathogenic bacteria

• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 265-271

Dates

published

2017

received

2016-06-15

revised

2016-08-08

accepted

2017-02-13

(unknown)

2017-04-14

Contributors

author

Alfredo Aires

• Centre for the Research and Technology for Agro-Environment and Biological Sciences, CITAB, Universidade de Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados, Portugal

author

Carla Dias

• Animal and Veterinary research Centre, CECAV, Universidade de Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados, Portugal

author

Rosa Carvalho

• Agronomy Department, Universidade de Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados, Portugal

author

Maria Saavedra

• Animal and Veterinary research Centre, CECAV, Universidade de Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados, Portugal

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Identifiers

DOI

10.18388/abp.2016_1374