Analysis of glycosylated flavonoids extracted from sweet-cherry stems, as antibacterial agents against pathogenic Escherichia coli isolates

• Authors: Alfredo Aires , Carla Dias , Rosa Carvalho , Maria Saavedra
• Languages of publication: EN

Abstracts

EN

The aim of this study was to evaluate the bioactivity of flavonoids extracted from sweet-cherry stems which are often used by a traditional system of medicine to treat gastro-intestinal and urinary tract infections but lacking any consistent scientific evidence; moreover the information about the class of phenolics, their content and the potential bioactivity of such material is very scarce. Thus, in this context, we have set a research study in which we evaluated the profile and content of phenolics extracted from sweet-cherry stems through a conventional (70ºC and 20 min) and ultrasound assisted extraction (40 kHz, room temperature and 20 min). The extracts were phytochemically characterized by using an HPLC-DAD-UV/VIS system and assayed by an in vitro minimum inhibitory concentration (MIC) bioassay against Escherichia coli isolates. Simultaneously, the total antioxidant activities were measured using the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS•+) radical cation assay. Our results indicate that sweet-cherry stems have a high content of sakuranetin, ferulic acid, p-coumaric acid, p-coumaroylquinic acid, chlorogenic acid and its isomer neochlorogenic acid. Their average levels were highly affected by the extraction method used (p<0.001). The same trend was observed for total antioxidant activity and MIC values. The extracts produced with ultrasounds presented both, a higher total antioxidant activity and a lower minimum inhibitory concentration. Statistical analyses of our results showed a significant correlation (p<0.01) of total antioxidant activity and minimum inhibitory concentration with phenolics present in the extracts studied. Thus, we can conclude that cherry stems can be further exploited to purify compounds and produce coproducts with enhanced biologically added value for pharmaceutical industry.

Keywords

EN

flavonoids   agro-food wastes   enhanced extraction   antimicrobial agents   pathogenic bacteria  

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 265-271

Dates

published

2017

received

2016-06-15

revised

2016-08-08

accepted

2017-02-13

(unknown)

2017-04-14

References

• Ajil CM, Brar SK, Verma M, Tyagi RD, Godbout S, Valéro JR (2012) Bio-processing of agro-byproducts to animal feed. Crit Rev Biotechnol 32: 382-400. doi: 10.3109/07388551.2012.659172.
• Alfieri M, Hidalgo A, Berardo N, Redaelli RJ (2014) Carotenoid composition and heterotic effect in selected Italian maize germplasm. Cereal Sci 59: 181-188. doi: 10.1016/j.jcs.2013.12.010
• Alighourchi HR, Barzegar M, Sahari MA, Abbasi S (2013) Effect of sonication on anthocyanins, total phenolic content, and antioxidant capacity of pomegranate juices. Int Food Res J 20: 1703-1709
• Ashokkumar M, Sunartio D, Kentish S, Mawson R, Simons L, Vilkhu K, Versteeg C K (2008) Modification of food ingredients by ultrasound to improve functionality: A preliminary study on a model system. Innov Food Sci Emerg Technol 9: 155-160. doi: 10.1016/j.ifset.2007.05.005
• Ballistreri G, Continella A, Gentile A, Amenta M, Fabroni S, Rapisarda P (2013) Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy. Food Chem 140: 630-638. doi: 10.1016/j.foodchem.2012.11.024.
• Bansode RR, Randolph P, Hurley S, Ahmedna M (2012) Evaluation of hypolipidemic effects of peanut skin-derived polyphenols in rats on Western-diet. Food Chem 135: 1659-1666. doi: 10.1016/j.foodchem.2012.06.034.
• Coates AR, Halls G, Hu Y (2011) Novel classes of antibiotics or more of the same? Br J Pharmacol 163: 184-194. doi: 10.1111/j.1476-5381.2011.01250.x.
• Da Silva GJ, Mendonca N (2012) Association between antimicrobial resistance and virulence in Escherichia coli. Virulence 3: 18-28. doi: 10.4161/viru.3.1.18382.
• Denev P, Kratchanova M, Ciz M, Lojek A, Vasicek O, Nedelcheva P, Blazheva D, Toshkova R, Gardeva E, Yossifova L, Hyrsl P, Vojtek L (2014) Biological activities of selected polyphenol-rich fruits related to immunity and gastrointestinal health. Food Chem 157: 37-44. doi: 10.1016/j.foodchem.2014.02.022.
• Fernando IDNS, Abeysinghe DC, Dharmadasa RM (2013) Determination of phenolic contents and antioxidant capacity of different parts of Withania somnifera (L.) Dunal from three different growth stages. Ind Crop Prod 50: 537-539. doi: 10.1016/j.indcrop.2013.08.042
• Jorgensen JH, Turnidge JD, Washington JA (2009) Antibacterial susceptibility tests: dilution and disk diffusion methods In Manual of clinical microbiology, 7th edn, Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH eds, pp 1526e154
• Kuete V, Alibert-Franco S, Eyong KO, Ngameni B, Folefoc GN, Nguemeving JR, Tangmouo JG, Fotso GW, Komguem J, Ouahouo BM, Bolla, JM, Chevalier J, Ngadjui BT, Nkengfack AE, Pages JM (2011) Antibacterial activity of some natural products against bacteria expressing a multidrug-resistant phenotype. Int J Antimicrob Agents 37: 156-161. doi: 10.1016/j.ijantimicag.2010.10.020.
• Lobo V, Patil A, Phatak A, Chandra N (2012) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4: 118-126. doi: 10.4103/0973-7847.70902.
• Murray PR, Boron EJ, Pfaller MA, Tenover F, Yolken RH (1999) ASM Press: Washington, DC pp 876
• Park HL, Yoo Y, Hahn TR, Bhoo SH, Lee SW, Cho MH (2014) Antimicrobial activity of UV-induced phenylamides from rice leaves. Molecules 19: 18139-18151. doi: 10.3390/molecules191118139.
• Sánchez-Vioque R, Polissiou M, Astraka K, de los Mozos-Pascual M, Tarantilis P, Herraiz-Peñalver D, Santana-Méridas O (2013) Polyphenol composition and antioxidant and metal chelating activities of the solid residues from the essential oil industry. Ind Crop Prod 49: 150-159. doi: 10.1016/j.indcrop.2013.04.053
• Sargın SA, Akçicek E, Selvi S (2013) An ethnobotanical study of medicinal plants used by the local people of Alaşehir (Manisa) in Turkey. J Ethnopharmacol 150: 860-874. doi: 10.1016/j.jep.2013.09.040.
• Sarker SD, Nahar L, Kumarasamy Y (2007) Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42: 321-324. doi: 10.1016/j.ymeth.2007.01.006.
• Schneider Y, Zahn S, Hofmann J, Wecks M, Rohm H (2006) Acoustic cavitation induced by ultrasonic cutting devices: A preliminary study. Ultrason Sonochem 13: 117-120. doi: 10.1016/j.ultsonch.2005.09.002.
• Schoevaerdts D, Bogaerts P, Grimmelprez A, de Saint-Hubert M, Delaere B, Jamart J, Swine C, Glupczynski Y (2011) Clinical profiles of patients colonized or infected with extended-spectrum beta-lactamase producing Enterobacteriaceae isolates: a 20 month retrospective study at a Belgian University Hospital. BMC Infect Dis 11: 12. doi: 10.1186/1471-2334-11-12.
• Shimizu T, Lin F, Hasegawa M, Nojiri H, Yamane H, Okada K (2012) The potential bioproduction of the pharmaceutical agent sakuranetin, a flavonoid phytoalexin in rice. Bioengineered 3: 352-357. doi: 10.4161/bioe.21546.
• Simões M, Bennett RN, Rosa EA (2009) Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Nat Prod Rep 26: 746-757. doi: 10.1039/b821648g.
• Tiwari BK, Patras A, Brunton N, Cullen PJ, O'Donnell CP (2010) Ultrasound assisted extraction of bioactive compounds from Nephelium lappaceum L. fruit peel using central composite face centered response surface design. Ultrason Sonochem 17: 598-604. doi: 10.1016/j.arabjc.2013.02.007
• Tsai SJ, Huang CS, Mong MC, Kam WY, Huang HY, Yin MC (2012) Anti-inflammatory and antifibrotic effects of naringenin in diabetic mice. J Agric Food Chem 60: 514-521. doi: 10.1021/jf203259h.
• Ultee A, Bennik MH, Moezelaar R (2002) The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 68: 1561-1568. doi: 10.1128/AEM.68.4.1561-1568.2002.
• Wong SL, Chang HS, Wang GJ, Chiang MY, Huang HY, Chen CH, Tsai SC, Lin CH, Chen IS (2011) Secondary metabolites from the roots of Neolitsea daibuensis and their anti-inflammatory activity. J Nat Prod 74: 2489-2496. doi: 10.1021/np100874f.

Identifiers

DOI

10.18388/abp.2016_1374