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2017 | 64 | 2 | 245-253
Article title

The use of serine protease from Yarrowia lipolytica yeast in the production of biopeptides from denatured egg white proteins

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EN
Abstracts
EN
Deriving non-conventional enzymes from cheaper sources than those used for commercially available enzymes may result in the production of hydrolysates with beneficial features, while drastically reducing the cost of hydrolysis. This is especially significant for enzymatic hydrolysis as a method of protein waste utilization. We have previously described the ability of non-commercial serine protease from Yarrowia lipolytica yeast to produce/release bioactive peptides from egg white protein by-products (EP). The enzymatic hydrolysis of EP was carried out for 24 h using the serine protease at an enzyme: substrate ratio of 1:30 (w/w). The obtained hydrolysate was characterized by protein degradation of 38% and also exhibited an antioxidant and cytokine-inducing activity. The isolation procedure (ultrafiltration and RP-HPLC) of bioactive peptides from the EP hydrolysate provided peptide fractions with significant antioxidant and ACE inhibitory activities. Three homogeneous and three heterogeneous peptide fractions were identified using MALDI-TOF/MS and the Mascot Search Results database. The peptides, mainly derived from ovalbumin, were composed of 2-19 amino-acid residues. We have thus demonstrated a novel ability of serine protease from Y. lipolytica to release biopeptides from an EP by-product.
Publisher

Year
Volume
64
Issue
2
Pages
245-253
Physical description
Dates
published
2017
received
2016-05-12
revised
2016-09-29
accepted
2016-10-17
(unknown)
2017-04-07
Contributors
author
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
  • Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
author
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
  • Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
  • Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv64p245kz
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