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2017 | 64 | 1 | 195-198
Article title

Modification of the deoxyribose test to detect strong iron binding

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Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+ complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+ in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe2+ concentration, at a constant concentration of a chelating agent.
Physical description
  • Bhat VB, Sridhar GR, Madyastha KM (2001) Efficient scavenging of hydroxyl radicals and inhibition of lipid peroxidation by novel analogues of 1,3,7-trimethyluric acid. Life Sci 70: 381-393.
  • Chobot V (2010) Simultaneous detection of pro- and antioxidative effects in the variants of the deoxyribose degradation assay. J Agric Food Chem 58: 2088-2094. doi: 10.1021/jf902395k.
  • De S, Adhikari S, Tilak-Jain J, Menon VP, Devasagayam TP (2008) Antioxidant activity of an aminothiazole compound: possible mechanisms. Chem Biol Interact 173: 215-223. doi: 10.1016/j.cbi.2008.03.011.
  • Dinis TC, Maderia VM, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315: 161-169.
  • Genaro-Mattos TC, Dalvi LT, Oliveira RG, Ginani JS, Hermes-Lima M (2009) Reevaluation of the 2-deoxyribose assay for determination of free radical formation. Biochim Biophys Acta 1790: 1636-1642. doi: 10.1016/j.bbagen.2009.09.003.
  • Guedes AC, Gião MS, Seabra R, Ferreira AC, Tamagnini P, Moradas-Ferreira P, Malcata FX (2013) Evaluation of the antioxidant activity of cell extracts from microalgae. Mar Drugs 11: 1256-1270. doi: 10.3390/md11041256.
  • Gutteridge JM, Halliwell B (1982) The role of the superoxide and hydroxyl radicals in the degradation of DNA and deoxyribose induced by a copper-phenanthroline complex. Biochem Pharmacol 31: 2801-2805.
  • Halliwell B, Gutteridge JM, Aruoma OI (1987) The deoxyribose method: a simple 'test-tube' assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 165: 215-219.
  • Halliwell B, Gutteridge JM (1981) Formation of thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. FEBS Lett 128: 347-352.
  • Khokhar S, Apenten RKO (2003) Iron binding characteristics of phenolic compounds: some tentative structure-activity relations. Food Chem 81: 133–140
  • Lapenna D, Ciofani G, Festi D, Neri M, Pierdomenico SD, Giamberardino MA, Cuccurullo F (2002) Antioxidant properties of ursodeoxycholic acid. Biochem Pharmacol 64: 1661-1667. doi: 10.1016/S0006-2952(02)01391-6.
  • Manoj VM, Aravindakumar CT (2003) Reaction of hydroxyl radicals with S-nitrosothiols: determination of rate constants and end product analysis. Org Biomol Chem 1: 1171-1175.
  • Mokdad-Bzeouich I, Kilani-Jaziri S, Mustapha N, Bedoui A, Ghedira K, Chekir-Ghedira L (2015) Evaluation of the antimutagenic, antigenotoxic, and antioxidant activities of Eriobotrya japonica leaves. Pharm Biol 53: 1786-1794. doi: 10.3109/13880209.2015.1008145.
  • Qian SY, Buettner GR (1999) Iron and dioxygen chemistry is an important route to initiation of biological free radical oxidations: an electron paramagnetic resonance spin trapping study. Free Radic Biol Med 26: 1447-1456.
  • Rachmilovich-Calis S, Meyerstein N, Meyerstein D (2009) A mechanistic study of the effects of antioxidants on the formation of malondialdehyde-like products in the reaction of hydroxyl radicals with deoxyribose. Chemistry 15: 7717-7723. doi: 10.1002/chem.200802272.
  • Saran M, Michel C, Stettmaier K, Bors W (2000) Arguments against the significance of the Fenton reaction contributing to signal pathways under in vivo conditions. Free Radic Res 33: 567-579.
  • White JM, Flashka HA (1973) An automated procedure, with use of ferrozine, for assay of serum iron and total iron-binding capacity. Clin Chem 19: 526-528.
  • Winterbourn C (1991) Factors that influence the deoxyribose oxidation assay for Fenton reaction products. Free Radic Biol Med 11: 353-360.
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