Tetraspanin CD151 mediates communication between PC3 prostate cancer cells and osteoblasts

• Authors: Alicja Grudowska , Dominika Czaplińska , Wojciech Polom , Marcin Matuszewski , Rafał Sądej , Andrzej Składanowski
• Languages of publication: EN

Abstracts

EN

Invasion and migration of cancer cells are crucial for the formation of secondary lesions. These require activation of signalling cascades modulated by the number of regulatory molecules. One such molecule is CD151, a member of evolutionary conserved tetraspanin family. CD151 is involved in cell adhesion, motility and cancer progression due to formation of complexes with laminin-binding integrins and regulation of growth factor receptors function (e.g. HGFR, TGFβR, EGFR). Recent studies point to correlation between CD151 expression and high tumour grade in prostate cancer (PCa). Herein, we investigated a possible role of CD151 in communication between PC3 cancer cells and either cancer-associated fibroblasts (CAFs) or osteoblasts, an interplay which is significant for metastasis. The analysis showed that although CAFs strongly enhanced both migration and invasion of PC3 prostate cancer cells, the effect was not dependent on CD151. On the other hand, CD151 was found to promote 3D migration as well as invasive growth in response to osteoblasts-secreted growth factors. Obtained data revealed that knockdown of CD151 abolished activation of pro-migratory/pro-survival kinases (i.e FAK, Src, HSP27) triggered by osteoblasts, along with expression of matrix metalloproteinase-13. This suggests that CD151 participates in communication between PC3 cells and bone microenvironment and the process can be considered as a significant step of PCa progression and metastasis.

Keywords

EN

CD151; prostate cancer; osteoblasts

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 1
• Pages: 135-141

Dates

published

2017

received

2016-06-04

revised

2016-07-19

accepted

2016-08-26

(unknown)

2017-03-14

Contributors

author

Alicja Grudowska

• Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland

author

Dominika Czaplińska

• Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland
• Department of Cell Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland

author

Wojciech Polom

• Department of Urology, Medical University of Gdansk, Gdańsk, Poland

author

Marcin Matuszewski

• Department of Urology, Medical University of Gdansk, Gdańsk, Poland

author

Rafał Sądej

• Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland

author

Andrzej Składanowski

• Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland

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Identifiers

DOI

10.18388/abp.2016_1356