Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27

• Authors: Ewelina Celińska , Agnieszka Drożdżyńska , Agnieszka Wita , Wojciech Juzwa , Wojciech Białas , Katarzyna Czaczyk , Włodzimierz Grajek
• Languages of publication: EN

Abstracts

EN

Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL-1), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL-1), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.

Keywords

EN

1,3-propanediol; gene knock-out; metabolic engineering; Enterobacteriaceae; group II intron; lactate dehydrogenase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 1
• Pages: 123-133

Dates

published

2017

(unknown)

2016-03-03

received

2016-05-19

revised

2016-07-01

accepted

2016-11-14

Contributors

author

Ewelina Celińska

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Agnieszka Drożdżyńska

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Agnieszka Wita

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Wojciech Juzwa

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Wojciech Białas

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Katarzyna Czaczyk

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Włodzimierz Grajek

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

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Identifiers

DOI

10.18388/abp.2016_1321