The structure of fadL mRNA and its interactions with RybB sRNA

• Authors: Agata Groszewska , Zuzanna Wroblewska , Mikolaj Olejniczak
• Languages of publication: EN

Abstracts

EN

Small bacterial RNAs (sRNAs) regulate translation by pairing with complementary sequences in their target mRNAs, in a process which is often dependent on the Hfq protein. Here, the secondary structure of a 95-nt long fragment of Salmonella fadL mRNA containing RybB sRNA binding site in the coding region was analyzed. The data indicated local rearrangements in this mRNA structure after the annealing of RybB. The filter retention data had shown that Hfq bound both RybB and the fadL mRNA fragment with tight affinities. Moreover, Hfq increased the rate of RybB annealing to fadL mRNA. These data indicate that Hfq directly participates in RybB interactions with the fadL mRNA.

Keywords

EN

Hfq; sRNA; mRNA; RybB; fadL; coding sequence

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 4
• Pages: 835-840

Dates

published

2016

received

2016-06-06

revised

2016-10-21

accepted

2016-10-24

(unknown)

2016-11-25

Contributors

author

Agata Groszewska

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Zuzanna Wroblewska

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Mikolaj Olejniczak

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

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Identifiers

DOI

10.18388/abp.2016_1361