Antiproliferative effect of β-escin - an in vitro study

• Authors: Gabriela Mojžišová , Martin Kello , Martina Pilátová , Vladimíra Tomečková , Janka Vašková , Ladislav Vaško , Silvia Bernátová , Ladislav Mirossay , Ján Mojžiš
• Languages of publication: EN

Abstracts

EN

This study examined the antiproliferative effects of β-escin (E) in cancer cells. The study showed that E inhibited cancer cells growth in a dose-dependent manner. The flow cytometric analysis revealed an escin-induced increase in the sub-G1 DNA content, which is considered to be a marker of apoptosis. Apoptosis was also confirmed by annexin V staining and DNA fragmentation assay. These effects were associated with increased generation of reactive oxygen species (ROS), caspase-3 activation and decreased mitochondrial membrane potential (MMP). Moreover, escin decreased mitochondrial protein content and mitochondrial fluorescence intensity as well as caused depletion of glutathione (GSH). However, activity of glutathione peroxidase (GPx) and glutathione reductase (GR) was not significantly changed in escin-treated cells. In conclusion, our results demonstrated that E has apoptotic effects in human cancer cells through the mechanisms involving mitochondrial perturbation. Although the exact mechanism needs to be investigated further, it can be concluded that E may be a useful candidate agent for cancer treatment.

Keywords

EN

escin; apoptosis; mitochondria; fluorescence fingerprint

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 1
• Pages: 79-87

Dates

published

2016

received

2015-03-22

revised

2015-11-05

accepted

2015-11-24

(unknown)

2016-01-27

Contributors

author

Gabriela Mojžišová

• Department of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Martin Kello

• Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Martina Pilátová

• Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Vladimíra Tomečková

• Department of Medical and Clinical Biochemistry, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Janka Vašková

• Department of Medical and Clinical Biochemistry, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Ladislav Vaško

• Department of Medical and Clinical Biochemistry, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Silvia Bernátová

• Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Ladislav Mirossay

• Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

author

Ján Mojžiš

• Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, 040 11 Košice, Slovak Republic

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Identifiers

DOI

10.18388/abp.2015_1013