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2016 | 63 | 1 | 79-87
Article title

Antiproliferative effect of β-escin - an in vitro study

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Abstracts
EN
This study examined the antiproliferative effects of β-escin (E) in cancer cells. The study showed that E inhibited cancer cells growth in a dose-dependent manner. The flow cytometric analysis revealed an escin-induced increase in the sub-G1 DNA content, which is considered to be a marker of apoptosis. Apoptosis was also confirmed by annexin V staining and DNA fragmentation assay. These effects were associated with increased generation of reactive oxygen species (ROS), caspase-3 activation and decreased mitochondrial membrane potential (MMP). Moreover, escin decreased mitochondrial protein content and mitochondrial fluorescence intensity as well as caused depletion of glutathione (GSH). However, activity of glutathione peroxidase (GPx) and glutathione reductase (GR) was not significantly changed in escin-treated cells. In conclusion, our results demonstrated that E has apoptotic effects in human cancer cells through the mechanisms involving mitochondrial perturbation. Although the exact mechanism needs to be investigated further, it can be concluded that E may be a useful candidate agent for cancer treatment.
Year
Volume
63
Issue
1
Pages
79-87
Physical description
Dates
published
2016
received
2015-03-22
revised
2015-11-05
accepted
2015-11-24
(unknown)
2016-01-27
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv63p79kz
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