New functionality of RNAComposer: an application to shape the axis of miR160 precursor structure

• Authors: Maciej Antczak , Mariusz Popenda , Tomasz Zok , Joanna Sarzynska , Tomasz Ratajczak , Katarzyna Tomczyk , Ryszard Adamiak , Marta Szachniuk
• Languages of publication: EN

Abstracts

EN

RNAComposer is a fully automated, web-interfaced system for RNA 3D structure prediction, freely available at http://rnacomposer.cs.put.poznan.pl/ and http://rnacomposer.ibch.poznan.pl/. Its main components are: manually curated database of RNA 3D structure elements, highly efficient computational engine and user-friendly web application. In this paper, we demonstrate how the latest additions to the system allow the user to significantly affect the process of 3D model composition on several computational levels. Although in general our method is based on the knowledge of secondary structure topology, currently the RNAComposer offers a choice of six incorporated programs for secondary structure prediction. It also allows to apply a conditional search in the database of 3D structure elements and introduce user-provided elements into the final 3D model. This new functionality contributes to a significant improvement of the predicted 3D model reliability and it facilitates a better model adjustment to the experimental data. This is exemplified based on the RNAComposer application for modelling of the 3D structures of precursors of the miR160 family members.

Keywords

EN

RNA 3D structure prediction; RNAComposer; plant miRNA precursor

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 4
• Pages: 737-744

Dates

published

2016

received

2016-05-30

revised

2016-06-22

accepted

2016-07-25

(unknown)

2016-10-14

Contributors

author

Maciej Antczak

• Institute of Computing Science and European Centre for Bioinformatics and Genomics, Poznan University of Technology, Poznań, Poland

author

Mariusz Popenda

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Tomasz Zok

• Institute of Computing Science and European Centre for Bioinformatics and Genomics, Poznan University of Technology, Poznań, Poland

author

Joanna Sarzynska

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

author

Tomasz Ratajczak

• Institute of Computing Science and European Centre for Bioinformatics and Genomics, Poznan University of Technology, Poznań, Poland

author

Katarzyna Tomczyk

• Institute of Computing Science and European Centre for Bioinformatics and Genomics, Poznan University of Technology, Poznań, Poland

author

Ryszard Adamiak

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
• Institute of Computing Science and European Centre for Bioinformatics and Genomics, Poznan University of Technology, Poznań, Poland

author

Marta Szachniuk

• Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland; Institute of Computing Science and European Centre for Bioinformatics and Genomics, Poznan University of Technology, Poznań, Poland

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Identifiers

DOI

10.18388/abp.2016_1329