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2016 | 63 | 3 | 589-593
Article title

Gastrodin ameliorates spinal cord injury via antioxidant and anti-inflammatory effects

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Abstracts
EN
Spinal cord injury (SCI) is one of the most severe traumatic injuries that results in dysfunction of limbs and trunk below the damaged section. Recent studies have shown that gastrodin (GAS) could improve the recovery of SCI. In the current study, we aimed to examine the possible mechanism underlying the effect of GAS on recovery of SCI in rats. In rats with SCI, GAS improved locomotor functions and decreased permeability of blood-spinal cord barrier, as illustrated by increase of Basso-Beattie-Bresnahan scores and decrease of Evans blue leakage. In addition, GAS inhibited inflammation, as evidenced by decrease of proinflammatory cytokines, including tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) in rats following SCI. Moreover, increase of TBARS content and decrease of glutathione (GSH) content and superoxide dismutase (SOD) activities in SCI rats were inhibited by GAS. Furthermore, GAS enhanced mRNA expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2), catalytic subunit of γ-glutamylcysteine ligase (GCLc) and modified subunit of γ-glutamylcysteine ligase (GCLm). The data suggested that GAS may promote the recovery of SCI through the enhancement of Nrf2-GCLc/GCLm signaling pathway, and subsequent improvement of oxidative stress and inflammation, resulting in decrease of permeability of BSCB and improved recovery of locomotor function in rats with SCI. The results have provided novel insights into GAS-related therapy of SCI and associated neurodegenerative diseases.
Publisher

Year
Volume
63
Issue
3
Pages
589-593
Physical description
Dates
published
2016
received
2016-02-12
revised
2016-03-17
accepted
2016-05-19
(unknown)
2016-07-30
Contributors
author
  • Department of Traumatic Orthopedics, The Second People's Hospital of Liaocheng, TaiShan Medical College, Liaocheng 252600, China
author
  • Department of Orthopedics, The People's Hospital of Gaotang, Liaocheng 252800, China
author
  • Department of Traumatic Orthopedics, The Second People's Hospital of Liaocheng, TaiShan Medical College, Liaocheng 252600, China
author
  • Department of Traumatic Orthopedics, The Second People's Hospital of Liaocheng, TaiShan Medical College, Liaocheng 252600, China
author
  • Department of Traumatic Orthopedics, The Second People's Hospital of Liaocheng, TaiShan Medical College, Liaocheng 252600, China
author
  • Department of Outpatient, The Sixth People's Hospital of Weifang, Weifang 261021, China
author
  • Department of Orthopedics, The Peking University Third Hospital, Beijing 100191, China
References
  • Alkabie S, Boileau AJ (2015) The role of therapeutic hypothermia after traumatic spinal cord injury-a systematic review. World Neurosurg 86: 432-449. doi: 10.1016/j.wneu.2015.09.079.
  • Allison DJ, Ditor DS (2015) Targeting inflammation to influence mood following spinal cord injury: a randomized clinical trial. J Neuroinflammation 12: 204. doi: 10.1186/s12974-015-0425-2.
  • Basso DM, Beattie MS, Bresnahan JC (1995) A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 12: 1-21. doi: 10.1089/neu.1995.12.1.
  • Benedict AL, Mountney A, Hurtado A, Bryan KE, Schnaar RL, Dinkova-Kostova AT, Talalay P (2012) Neuroprotective effects of sulforaphane after contusive spinal cord injury. J Neurotrauma 29: 2576-2586. doi: 10.1089/neu.2012.2474.
  • Chen MH, Liu YH, Xu H, Xu DW, Wang CN, Wang Y, Duan CW, Zhou Y, Kan P, Shen AG, Wang YH (2015) Lentiviral vector-mediated p27kip1 expression facilitates recovery after spinal cord injury. Mol Neurobiol doi: 10.1007/s12035-015-9498-2.
  • Dai JN, Zong Y, Zhong LM, Li YM, Zhang W, Bian LG, Ai QL, Liu YD, Sun J, Lu D (2011) Gastrodin inhibits expression of inducible NO synthase, cyclooxygenase-2 and proinflammatory cytokines in cultured LPS-stimulated microglia via MAPK pathways. PLoS One 6: e21891. doi: 10.1371/journal.pone.0021891.
  • Dionyssiotis Y, Mavrogenis A, Trovas G, Skarantavos G, Papathanasiou J, Papagelopoulos P (2014) Bone and soft tissue changes in patients with spinal cord injury and multiple sclerosis. Folia Med (Plovdiv) 56: 237-244. doi: 10.1515/folmed-2015-0002.
  • Edwards WB, Schnitzer TJ, Troy KL (2014) Bone mineral and stiffness loss at the distal femur and proximal tibia in acute spinal cord injury. Osteoporos Int 25: 1005-1015. doi: 10.1007/s00198-013-2557-5.
  • Evaniew N, Belley-Cote EP, Fallah N, Noonan V, Rivers CS, Dvorak MF (2015) Methylprednisolone for the treatment of patients with acute spinal cord injuries: A systematic review and meta-analysis. J Neurotrauma doi: 10.1089/neu.2015.4192.
  • Ewan EE, Hagg T (2015) Intrathecal acetyl-l-carnitine protects tissue and improves function after a mild contusive spinal cord injury in rats. J Neurotrauma doi: 10.1089/neu.2015.4030.
  • Giuliano F, Hultling C, El MW, Smith MD, Osterloh IH, Orr M, Maytom M (1999) Randomized trial of sildenafil for the treatment of erectile dysfunction in spinal cord injury. Sildenafil Study Group. Ann Neurol 46: 15-21.
  • Guan D, Su Y, Li Y, Wu C, Meng Y, Peng X, Cui Y (2015) Tetramethylpyrazine inhibits CoCl2 -induced neurotoxicity through enhancement of Nrf2/GCLc/GSH and suppression of HIF1alpha/NOX2/ROS pathways. J Neurochem 134: 551-565. doi: 10.1111/jnc.13161.
  • Haan N, Zhu B, Wang J, Wei X, Song B (2015) Crosstalk between macrophages and astrocytes affects proliferation, reactive phenotype and inflammatory response, suggesting a role during reactive gliosis following spinal cord injury. J Neuroinflammation 109. doi: 10.1186/s12974-015-0327-3.
  • Hayashi M, Ueyama T, Tamaki T, Senba E (1997) Expression of neurotrophin and IL-1 beta mRNAs following spinal cord injury and the effects of methylprednisolone treatment. Kaibogaku Zasshi 72: 209-213.
  • Henke D, Gorgas D, Doherr MG, Howard J, Forterre F, Vandevelde M (2015) Longitudinal extension of myelomalacia by intramedullary and subdural haemorrhage in a canine model of spinal cord injury. Spine J doi: 10.1016/j.spinee.2015.09.018.
  • Hu J, Lang Y, Cao Y, Zhang T, Lu H (2015) The neuroprotective effect of tetramethylpyrazine against contusive spinal cord injury by activating PGC-1alpha in rats. Neurochem Res 40: 1393-1401. doi: 10.1007/s11064-015-1606-1.
  • Hu JZ, Huang JH, Xiao ZM, Li JH, Li XM, Lu HB (2013) Tetramethylpyrazine accelerates the function recovery of traumatic spinal cord in rat model by attenuating inflammation. J Neurol Sci 324: 94-99. doi: 10.1016/j.jns.2012.10.009.
  • Khayrullina G, Bermudez S, Byrnes KR (2015) Inhibition of NOX2 reduces locomotor impairment, inflammation, and oxidative stress after spinal cord injury. J Neuroinflammation 172. doi: 10.1186/s12974-015-0391-8.
  • Kieseier BC, Wiendl H (2015) Nrf2 and beyond: deciphering the mode of action of fumarates in the inflamed central nervous system. Acta Neuropathol 130: 297-298. doi: 10.1007/s00401-015-1457-5.
  • Kleibeuker W, Gabay E, Kavelaars A, Zijlstra J, Wolf G, Ziv N, Yirmiya R, Shavit Y, Tal M, Heijnen CJ (2008) IL-1 beta signaling is required for mechanical allodynia induced by nerve injury and for the ensuing reduction in spinal cord neuronal GRK2. Brain Behav Immun 22: 200-208. doi: 10.1016/j.bbi.2007.07.009.
  • Li C, Chen X, Zhang N, Song Y, Mu Y (2012) Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats. Neural Regen Res 7: 325-331. doi: 10.3969/j.issn.1673-5374.2012.05.001.
  • Luo Y, Fu C, Wang Z, Zhang Z, Wang H, Liu Y (2015) Mangiferin attenuates contusive spinal cord injury in rats through the regulation of oxidative stress, inflammation and the Bcl2 and Bax pathway. Mol Med Rep 12: 7132-7138. doi: 10.3892/mmr.2015.4274.
  • Lv R, Mao N, Wu J, Lu C, Ding M, Gu X, Wu Y, Shi Z (2015) Neuroprotective effect of allicin in a rat model of acute spinal cord injury. Life Sci 143: 114-123. doi: 10.1016/j.lfs.2015.11.001.
  • Park S, Kim DS, Kang S (2011) Gastrodia elata Blume water extracts improve insulin resistance by decreasing body fat in diet-induced obese rats: vanillin and 4-hydroxybenzaldehyde are the bioactive candidates. Eur J Nutr 50: 107-118. doi: 10.1007/s00394-010-0120-0.
  • Peng Z, Wang H, Zhang R, Chen Y, Xue F, Nie H, Chen Y, Wu D, Wang Y, Wang H, Tan Q (2013) Gastrodin ameliorates anxiety-like behaviors and inhibits IL-1beta level and p38 MAPK phosphorylation of hippocampus in the rat model of posttraumatic stress disorder. Physiol Res 62: 537-545.
  • Peng Z, Wang S, Chen G, Cai M, Liu R, Deng J, Liu J, Zhang T, Tan Q, Hai C (2015) Gastrodin alleviates cerebral ischemic damage in mice by improving anti-oxidant and anti-inflammation activities and inhibiting apoptosis pathway. Neurochem Res 40: 661-673. doi: 10.1007/s11064-015-1513-5.
  • Sandberg M, Patil J, D'Angelo B, Weber SG, Mallard C (2014) NRF2-regulation in brain health and disease: implication of cerebral inflammation. Neuropharmacology 298-306. doi: 10.1016/j.neuropharm.2013.11.004.
  • Sharma HS (2011) Early microvascular reactions and blood-spinal cord barrier disruption are instrumental in pathophysiology of spinal cord injury and repair: novel therapeutic strategies including nanowired drug delivery to enhance neuroprotection. J Neural Transm (Vienna) 118: 155-176. doi: 10.1007/s00702-010-0514-4.
  • Song C, Fang S, Lv G, Mei X (2013) Gastrodin promotes the secretion of brain-derived neurotrophic factor in the injured spinal cord. Neural Regen Res 8: 1383-1389. doi: 10.3969/j.issn.1673-5374.2013.15.005.
  • Sothmann J, Stander J, Kruger N, Dunn R (2015) Epidemiology of acute spinal cord injuries in the Groote Schuur Hospital Acute Spinal Cord Injury (GSH ASCI) Unit, Cape Town, South Africa, over the past 11 years. S Afr Med J 105: 835-839. doi: 10.7196/SAMJnew.8072.
  • Sun XF, Wang W, Wang DQ, Du GY (2004) Research progress of neuroprotective mechanisms of Gastrodia elata and its preparation. Zhongguo Zhong Yao Za Zhi 29: 292-295.
  • Tyagi P, Kadekawa K, Kashyap M, Pore S, Yoshimura N (2015) Spontaneous recovery of reflex voiding following spinal cord injury mediated by anti-inflammatory and neuro-protective factors. Urology 88: 57-65. doi: 10.1016/j.urology.2015.10.017.
  • Wang W, Shen H, Xie JJ, Ling J, Lu H (2015) Neuroprotective effect of ginseng against spinal cord injury induced oxidative stress and inflammatory responses. Int J Clin Exp Med 8: 3514-3521.
  • Wang X, Campos CR, Peart JC, Smith LK, Boni JL, Cannon RE, Miller DS (2014) Nrf2 upregulates ATP binding cassette transporter expression and activity at the blood-brain and blood-spinal cord barriers. J Neurosci 34: 8585-8593. doi: 10.1523/JNEUROSCI.2935-13.2014.
  • Wang XL, Xing GH, Hong B, Li XM, Zou Y, Zhang XJ, Dong MX (2014) Gastrodin prevents motor deficits and oxidative stress in the MPTP mouse model of Parkinson's disease: involvement of ERK1/2-Nrf2 signaling pathway. Life Sci 114: 77-85. doi: 10.1016/j.lfs.2014.08.004.
  • Winkler EA, Sengillo JD, Sagare AP, Zhao Z, Ma Q, Zuniga E, Wang Y, Zhong Z, Sullivan JS, Griffin JH, Cleveland DW, Zlokovic BV (2014) Blood-spinal cord barrier disruption contributes to early motor-neuron degeneration in ALS-model mice. Proc Natl Acad Sci U S A 111: E1035-E1042. doi: 10.1073/pnas.1401595111.
  • Xu X, Lu Y, Bie X (2007) Protective effects of gastrodin on hypoxia-induced toxicity in primary cultures of rat cortical neurons. Planta Med 73: 650-654. doi: 10.1055/s-2007-981523.
  • Yang T, Wu L, Wang H, Fang J, Yao N, Xu Y (2015) Inflammation level after decompression surgery for a rat model of chronic severe spinal cord compression and effects on ischemia-reperfusion injury. Neurol Med Chir (Tokyo) 55: 578-586. doi: 10.2176/nmc.oa.2015-0022.
  • Yu DS, Cao Y, Mei XF, Wang YF, Fan ZK, Wang YS, Lv G (2014) Curcumin improves the integrity of blood-spinal cord barrier after compressive spinal cord injury in rats. J Neurol Sci 346: 51-59. doi: 10.1016/j.jns.2014.07.056.
  • Yu DS, Liu LB, Cao Y, Wang YS, Bi YL, Wei ZJ, Tong SM, Lv G, Mei XF (2015) Combining bone marrow stromal cells with green tea polyphenols attenuates the blood-spinal cord barrier permeability in rats with compression spinal cord injury. J Mol Neurosci 56: 388-396. doi: 10.1007/s12031-015-0564-z.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv63p589kz
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