Screening and characterization of thermo-active enzymes of biotechnological interest produced by thermophilic Bacillus isolated from hot springs in Tunisia

• Authors: Wajdi Thebti , Yosra Riahi , Rawand Gharsalli , Omrane Belhadj
• Languages of publication: EN

Abstracts

EN

As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.

Keywords

EN

temperature; amylase; protease; cellulase; xylanase; mannanase; Geobacillus; Tunisian hot springs

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 3
• Pages: 581-587

Dates

published

2016

received

2016-02-10

revised

2016-03-31

accepted

2016-04-29

(unknown)

2016-07-06

Contributors

author

Wajdi Thebti

• Laboratory of Biochemistry & Technobiology, Faculty of Science of Tunis, University of Tunis El Manar, Campus University Farhat HACHED El Manar 2092, Tunis

author

Yosra Riahi

• Laboratory of Biochemistry & Technobiology, Faculty of Science of Tunis, University of Tunis El Manar, Campus University Farhat HACHED El Manar 2092, Tunis

author

Rawand Gharsalli

• Laboratory of Biochemistry & Technobiology, Faculty of Science of Tunis, University of Tunis El Manar, Campus University Farhat HACHED El Manar 2092, Tunis

author

Omrane Belhadj

• Laboratory of Biochemistry & Technobiology, Faculty of Science of Tunis, University of Tunis El Manar, Campus University Farhat HACHED El Manar 2092, Tunis

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Identifiers

DOI

10.18388/abp.2016_1271