Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

PL EN


Preferences help
enabled [disable] Abstract
Number of results
2016 | 63 | 3 | 577-580

Article title

Genetic polymorphism of CSN2 gene in Banat White and Carpatina goats

Content

Title variants

Languages of publication

EN

Abstracts

EN
The main objective of the current study was to obtain preliminary results on genetic polymorhism of A and C variants at the CSN2 locus in indigenous Romanian goat (Banat White, Carpatina) breeds using a rapid and efficient genotyping method, TaqMan assay (Applied Biosystems, USA). Hair follicle samples were taken from 73 Banat White and 82 Carpatina purebred goats from Arad, Caras-Severin and Timis counties. After the optimization of the genotyping assay it was found that the most frequent allele at the CSN2 locus was C in Banat White breed, while CSN2*A and CSN2*C showed similar frequencies in Carpatina breed (0.51 and 0.49, respectively). All three genotypes were detected in the two studied breeds, however, AA was the least frequent, especially in Banat White. The studied polymorphisms are potential markers for milk production in the studied breeds and the results will be useful in future works aimed at identifying possible associations with milk production traits, in order to test the feasibility of producing hypoallergenic organic goat milk.

Year

Volume

63

Issue

3

Pages

577-580

Physical description

Dates

published
2016
received
2016-02-10
revised
2016-03-24
accepted
2016-05-18
(unknown)
2016-07-06

Contributors

author
  • Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, Caransebes, Romania
  • Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, Debrecen, Hungary
author
  • Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, Caransebes, Romania
  • Research and Development Station for Bovine Arad, Academy for Agricultural and Forestry Sciences,Arad, Romania
author
  • Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, Caransebes, Romania
  • Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, Debrecen, Hungary
author
  • Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, Caransebes, Romania
  • Research and Development Station for Sheep and Goats Caransebes, Academy for Agricultural and Forestry Sciences, Caransebes, Romania

References

  • Caroli A, Chiatti F, Chessa S, Rignanese D, Bolla P, Pagnacco G (2006) Focusing on the goat casein gene complex. J Dairy Sci 89: 3178-3187. http:// doi: 10.3168/jds.S0022-0302(06)72592-9.
  • Chessa S, Budelli E, Chiatti AM, Cito P, Bolla P, Caroli A (2005) Short communication: predominance of β-casein (CSN2) C allele in goat breeds reared in Italy. J Dairy Sci 88: 1878-1881. doi: 10.3168/jds.S0022-0302(05)72863-0.
  • Chessa S, Rignanese F, Küpper J, Pagnacco G, Erhardt G, Caroli A (2008) Short communication: the β-casein (CSN2) silent allele C1 is highly spread in goat breeds. J Dairy Sci 91: 4433-4436. doi: 10.3168/jds.2008-1228.
  • Chianese L, Caira S, Garro G, Quarto M, Mauriello R, Addeo F (2007) Occurrence of genetic polymorphism at goat β-CN locus. Proc. 5th Int. Symp. Challenge to Sheep and Goats Milk Sectors, p 69. Alghero, Italy.
  • Cosenza G, Pauciullo A, Gallo D, Berardino DD, Ramunno L (2005) A Ssp I PCR-RFLP detecting a silent allele at the goat CSN2 locus. J Dairy Res 72: 456-459. doi: 10.1017/S0022029905001342.
  • Dragomir N (2009) Multifunctional utilization of pastures in Romania. Sci Pap Anim Sci Biotech 42: 191-194.
  • FAO/IAEA (2004) Agriculture Biotechnology Laboratory - Handbook of Laboratory Exercises. IAEA Laboratories, p 18. Seibersdorf, Austria.
  • Galliano F, Saletti R, Cunsolo V, Foti S, Marletta D, Bordonaro S, D'Urso G (2004) Identification and characterization of a new β-casein variant in goat milk by high-performance liquid chromatography with electrospray ionization mass spectrometry and mass-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom 18: 1972-1982.
  • Gigli I, Maizon DO, Riggio V, Sardina MT, Portolano B (2008) Short communication: casein haplotype variability in Sicilian dairy goat breeds. J Dairy Sci 91: 3687-3692. doi: 10.3168/jds.2008-106.
  • Hayes H, Petit E, Bouniol C, Popescu P (1993) Localization of the alpha-S2-casein gene (CASAS2) to the homologous cattle, sheep and goat chromosome 4 by in situ hybridization. Cytogenet Cell Genet 64: 281-285. doi: 10.1159/000133593.
  • Mahé MF, Grosclaude F (1993) Polymorphism of β-casein in Creole goat of Guadaloupe, evidence for a null allele. Genet Sel Evol 25: 403-408.
  • Martin P, Szymanowska M, Zwierzchowski L, Leroux C (2002) The impact of genetic polymorphisms on the protein composition of ruminant milks. Reprod Nutr Dev 42: 433-459. doi: 10.1051/rnd:2002036.
  • Neveu C, Mollé D, Moreno J, Martin P, Léonil J (2002) Heterogeneity of caprine beta-casein elucidated by RP-HPLC/MS: genetic variants and phosphorylations. J Protein Chem 21: 557-567.
  • Padeanu I (2001) Tehnologia cresterii ovinelor si caprinelor. Mirton ?. ed, p 375. Timisoara (Language?).
  • Pascal C, Padeanu I, Calin I, Daraban S, Nacu G (2011) Researches related to meat yield aptitudes of Carpatina breed reared in Romania. Sci Pap Anim Sci Biotech 55: 328-331.
  • Pascal C (2006) The morphologic and productive specific parameters of goats reared in North-Eastern area of Romania. In International Symposium on Goat Farming in Central and Eastern European Countries: Present and Future - IGA Regional Conference, Constanta, Romania, June 27-30. 2006,. http://www.iga-goatworld.org/.
  • Pascal C, Confederat M (2006) Researchers with reference at the quantitative and qualitative milk production at indigenous local goats growled and exploited in the North-Estern area of Romania. In International Symposium on Goat Farming in Central and Eastern European Countries: Present and Future - IGA Regional Conference, Constanta, Romania, June 27-30, 2006. http://www.iga-goatworld.org/.
  • Persuy MA, Printz C, Medrano JF, Mercier JC (1999) A single nucleotide deletion resulting in a premature stop codon is associated with marked reduction of transcripts from a goat β-casein null allele.Anim Genet 30: 444-451. doi: 10.1046/j.1365-2052.1999.00547.x.
  • Raducuta I, Calin I, Purdoiu S (2012) The evolution and current situation of goat breeding in Romania. Sci Pap Anim Sci Biotech 55: 223-226.
  • Ramunno L, Mariani P, Pappalardo M, Rando A, Capuano M, Di Gregorio P, Cosenza G (1995) Un gene ad effetto maggiore sul contenuto di caseina β nel lattedi capra. pp 186-186 in Proc. 11th ASPANat. Congress, Grado, Italy (in Italian?).
  • Rijnkels M (2002) Multispecies comparison of the casein gene loci and evolution of casein gene family. J Mammary Gland Biol Neoplasia 7: 327-345. doi: 10.1023/A:1022808918013.
  • Roberts B, DiTullio P, Vitale J, Hehir K, Gordon K (1992) Cloning of the goat beta-casein-encoding gene and expression in transgenic mice. Gene 121: 255-262. doi: 10.1016/0378-1119(92)90129-D.
  • Sacchi P, Chessa S, Budelli E, Bolla P, Ceriotti G, Soglia D, Rasero R, Cauvin E, Caroli A (2005) Casein haplotype structure in five italian goat breeds. J Dairy Sci 88: 1561-1568. doi: 10.3168/jds.S0022-0302(05)72825-3.
  • Sztankóová Z, Kysel'ova J, Kott T, Kottova E (2008) Technical note: detection of the C allele of β-casein (CSN2) in Czech dairy goat breeds using Light Cycler analysis. J Dairy Sci 91: 4053-4057. doi: 10.3168/jds.2007-0747.
  • Tortorici L, Di Gerlando R, Mastrangelo S, Sardina MT, Portolano B (2014) Genetic characterisation of CSN2 gene in Girgentana goat breed. Italian J Anim Sci 13: 720-722. doi: 10.4081/ijas.2014.3414.
  • Vacca GM, Ben Ali OA, Carcangiu V, Pazzola M, Dettori ML (2009) Genetic structure of the casein gene cluster in the Tunisian native goat breed. Small Rumin Res 87: 33-38. doi: 10.1016/j.smallrumres.2009.09.034.
  • Vinesh PV, Biswajit B, Rupinder K, Kumar DT, Lal GS, Sachinandan D (2013) Characterization of β-casein gene in Indian riverine buffalo. Gene 527: 683-688. doi: 10.1016/j.gene.2013.06.029.
  • Vlaic A, Balteanu V, Pop F, Carsai T, Suteu M (2010) Milk protein polymorphisms study in some Carpathian goat populations reared in the central part of Romania. Bulletin UASVM Animal Science and Biotechnologies 67: 54-59.
  • Voia S, Padeanu I, Daraban S, Mot T, Dronca D, Pet I, Gavojdian D, Ivan M (2010) Study regarding goat milk composition and the growth rate in kids of Carpatina goat breed. Sci Pap Anim Sci Biotech 43: 324-327.
  • Zaulet M, Kevorkian S, Enache M, Ghita E, Lazar C, Rebedea M (2008) Genetic polymorhism of some proteins in the milk of Carpathian goat. Zootehnie si Biotehnologii 41: 201-207.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv63p577kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.