Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

• Authors: Magdalena Jaszek , Justyna Miłek , Jerzy Żuchowski , Dawid Stefaniuk , Monika Prendecka
• Languages of publication: EN

Abstracts

EN

The effect of supplementation of medium with rapeseed meal (RM) on production of biotechnologically important enzymes was investigated in submerged cultures of the white rot fungus Cerrena unicolor. The addition of RM (3.5% w/v) distinctly stimulated the activities of laccase, chitinase, and β-glucosidase. As compared to the control, the activities of chitinase, β-glucosidase, and laccase in the RM supplemented cultures were up to 4.1, 8.4, and 3.9 times higher, respectively. The results of the spectrophotometric and spectrofluorometric measurements were additionally confirmed by zymographic analysis of the samples. The level of sugars and phenolic compounds as well as the antioxidative ability of fungal preparations were also determined. The results obtained indicate that the submerged liquid fermentation of rapeseed meal can be proposed as an inexpensive and very effective method for biotechnological production of chitinase, β-glucosidase, and laccase by C. unicolor.

Keywords

EN

Cerrena unicolor; laccase; chitinase; β-glucosidase; white rot fungi; rapeseed meal

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 3
• Pages: 549-554

Dates

published

2016

received

2015-12-01

revised

2016-03-04

accepted

2016-04-25

(unknown)

2016-06-02

Contributors

author

Magdalena Jaszek

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Justyna Miłek

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Jerzy Żuchowski

• Department of Biochemistry, Institute of Soil Science and Plant Cultivation, Puławy, Poland

author

Dawid Stefaniuk

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Monika Prendecka

• Chair and Department of Human Physiology, Medical University of Lublin, Lublin, Poland

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Identifiers

DOI

10.18388/abp.2015_1227