Expression profiles of MGMT, p16, and APC genes in tumor and matching surgical margin from patients with oral squamous cell carcinoma

• Authors: Joanna Strzelczyk , Karolina Gołąbek , Łukasz Krakowczyk , Aleksander Owczarek
• Languages of publication: EN

Abstracts

EN

The aim of the study was to assess the expression of MGMT, p16, and APC genes in tumors and matching surgical margin samples from 56 patients with primary OSCC. We also analyzed the association of the clinical variables with the expression of the studied genes. After RNA isolation and cDNA synthesis gene expression levels were assessed by quantitative reverse transcription (qRT)-PCR. Two-sided parametrical Student's t-test for independent groups with equal/unequal variances showed no statistically significant differences in genes' expression in tumor compared to margin samples. No association was found between the genes' expression and clinical parameters, except for MGMT, whose low expression was probably associated with smoking (0.87 vs 1.34, p=0.065). 'Field cancerization' is an area with genetically or epigenetically altered cells and at the same time a risk factor for cancer. Disturbances in gene expression could also be the source of damages leading to cancerization. In conclusion, it is important to mention that the field remaining after a surgery may pose an increased risk of cancer development. It may be suggested that the diagnosis and treatment of cancers should not be concentrated only on the tumor itself, but also on the cancer field effect. Therefore, further molecular analysis on surgical margins and additional research regarding their assessment are required.

Keywords

EN

HNSCC; gene expression; surgical margin; MGMT; p16; APC

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 3
• Pages: 505-509

Dates

published

2016

received

2015-10-20

revised

2016-03-04

accepted

2016-05-18

(unknown)

2016-08-05

Contributors

author

Joanna Strzelczyk

• Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Zabrze, Katowice, Poland

author

Karolina Gołąbek

• Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Zabrze, Katowice, Poland

author

Łukasz Krakowczyk

• Clinic of Oncological and Reconstructive Surgery, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland

author

Aleksander Owczarek

• Department of Statistics, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland

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Identifiers

DOI

10.18388/abp.2015_1204